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Journal of Histochemistry and Cytochemistry, Vol. 46, 787-792, July 1998, Copyright © 1998, The Histochemical Society, Inc.

RAPID COMMUNICATION

NADPH Diaphorase Histochemistry Detects Inducible Nitric Oxide Synthetase Activity in the Thymus of Naive and Staphylococcal Enterotoxin B-stimulated Mice

László Virága, György Haskóa, Andrew L. Salzmana, and Csaba Szabóa
a Division of Critical Care, Children's Hospital Medical Center, Cincinnati, Ohio

Correspondence to: Csaba Szabó, Div. of Critical Care, Children’s Hospital Medical Center, 3333 Burnet Ave., Cincinnati, OH 45229.

Here we examined the changes in NADPH diaphorase (NADPHd) and inducible nitric oxide synthetase (iNOS) positivity in the medulla of the mouse thymus in response to treatment with the superantigen, Staphylococcal enterotoxin B (SEB). A few NADPHd+ and iNOS+ cells scattered in the medulla were detected in the thymi of naive mice. SEB induced the appearance of a large number of NADPHd+- and iNOS-immunoreactive cells in the thymic medulla. In the thymus of iNOS-deficient mice, a total absence of these NADPHd+ and iNOS+ medullary cells was found both under basal conditions and after SEB stimulation. With the NADPHd reaction, only endothelial staining was detected in the thymi of iNOS-deficient mice. Our data indicate that NADPHd+ cells in the thymic medulla express iNOS and that SEB induces iNOS expression in the mouse thymus. (J Histochem Cytochem 46:787–791, 1998)

Key Words: NADPH diaphorase, nitric oxide synthetase, thymus, Staphylococcal enterotoxin B


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