Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sender, S.
Right arrow Articles by Gros, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sender, S.
Right arrow Articles by Gros, G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 46, 855-862, July 1998, Copyright © 1998, The Histochemical Society, Inc.

ARTICLE

Localization of Carbonic Anhydrase IV in Rat and Human Heart Muscle

Susanne Sendera, Brigitte Deckerb, Christiane D. Fenskec, William S. Slyd, Nicholas D. Carterc, and Gerolf Grosa
a Vegetative Physiologie, Zentrum Physiologie, Medizinische Hochschule Hannover, Hannover, Germany
b Zellbiologie und Elektronenmikroskopie, Zentrum Anatomie, Medizinische Hochschule Hannover, Hannover, Germany
c Medical Genetics Unit, St George's Hospital Medical School, London, United Kingdom
d Edward A. Doisy Department of Biochemistry and Molecular Biology, St Louis University School of Medicine, St Louis, Missouri

Correspondence to: Susanne Sender, Vegetative Physiologie, 4220, Medizinische Hochschule Hannover, 30623 Hannover, Germany.

We investigated carbonic anhydrase IV (CA IV) in rat and human heart with immunohistochemical methods by both light and electron microscopy. In cryosections that were incubated with anti-CA IV/FITC, the capillaries showed a strong reaction for CA IV. In paraffin and semithin sections treated with anti-CA IV/ABC (avidin–biotin–peroxidase complex) blood vessels, capillaries, and sarcolemma (SL) were positively stained. By staining ultrathin sections with anti-CA IV/immunogold, CA IV could also be demonstrated at the latter two locations, including the specialized sarcolemmal structures intercalated discs, and T-tubules. In addition, by this method CA IV was seen to be associated with the sarcoplasmic reticulum (SR). The absence of immunostaining in SR and/or SL with some techniques probably indicates a problem of accessibility of the antigenic sites. In line with the immunohistochemical results, CA IV mRNA expression was visualized in both endothelial and muscle cells by in situ hybridization histochemistry. (J Histochem Cytochem 46:855–861, 1998)

Key Words: carbonic anhydrase IV, capillaries, sarcolemma, sarcoplasmic reticulum, heart, skeletal muscle, immunohistochemistry, in situ hybridization


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
R. J. Scheibe, G. Gros, S. Parkkila, A. Waheed, J. H. Grubb, G. N. Shah, W. S. Sly, and P. Wetzel
Expression of Membrane-bound Carbonic Anhydrases IV, IX, and XIV in the Mouse Heart
J. Histochem. Cytochem., December 1, 2006; 54(12): 1379 - 1391.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. M. Purkerson and G. J. Schwartz
Expression of membrane-associated carbonic anhydrase isoforms IV, IX, XII, and XIV in the rabbit: induction of CA IV and IX during maturation
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2005; 288(5): R1256 - R1263.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
I Nishimori, E Miyaji, K Morimoto, K Nagao, M Kamada, and S Onishi
Serum antibodies to carbonic anhydrase IV in patients with autoimmune pancreatitis
Gut, February 1, 2005; 54(2): 274 - 281.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
P. P. Dzeja and A. Terzic
Phosphotransfer networks and cellular energetics
J. Exp. Biol., June 15, 2003; 206(12): 2039 - 2047.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Sterling, B. V. Alvarez, and J. R. Casey
The Extracellular Component of a Transport Metabolon. EXTRACELLULAR LOOP 4 OF THE HUMAN AE1 Cl-/HCO3- EXCHANGER BINDS CARBONIC ANHYDRASE IV
J. Biol. Chem., July 12, 2002; 277(28): 25239 - 25246.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
C. Geers and G. Gros
Carbon Dioxide Transport and Carbonic Anhydrase in Blood and Muscle
Physiol Rev, April 1, 2000; 80(2): 681 - 715.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. S. Knuppel-Ruppert, G. Gros, W. Harringer, and H.-P. Kubis
Immunochemical evidence for a unique GPI-anchored carbonic anhydrase isozyme in human cardiomyocytes
Am J Physiol Heart Circ Physiol, April 1, 2000; 278(4): H1335 - H1344.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
T Peters, F Papadopoulos, H. Kubis, and G Gros
Properties of a carbonic anhydrase inhibitor protein in flounder serum
J. Exp. Biol., January 10, 2000; 203(19): 3003 - 3009.
[Abstract] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1998

 
Purchase HCS Short Course Manual on HCS site