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Journal of Histochemistry and Cytochemistry, Vol. 46, 855-862, July 1998, Copyright © 1998, The Histochemical Society, Inc.

ARTICLE

Localization of Carbonic Anhydrase IV in Rat and Human Heart Muscle

Susanne Sendera, Brigitte Deckerb, Christiane D. Fenskec, William S. Slyd, Nicholas D. Carterc, and Gerolf Grosa
a Vegetative Physiologie, Zentrum Physiologie, Medizinische Hochschule Hannover, Hannover, Germany
b Zellbiologie und Elektronenmikroskopie, Zentrum Anatomie, Medizinische Hochschule Hannover, Hannover, Germany
c Medical Genetics Unit, St George's Hospital Medical School, London, United Kingdom
d Edward A. Doisy Department of Biochemistry and Molecular Biology, St Louis University School of Medicine, St Louis, Missouri

Correspondence to: Susanne Sender, Vegetative Physiologie, 4220, Medizinische Hochschule Hannover, 30623 Hannover, Germany.

We investigated carbonic anhydrase IV (CA IV) in rat and human heart with immunohistochemical methods by both light and electron microscopy. In cryosections that were incubated with anti-CA IV/FITC, the capillaries showed a strong reaction for CA IV. In paraffin and semithin sections treated with anti-CA IV/ABC (avidin–biotin–peroxidase complex) blood vessels, capillaries, and sarcolemma (SL) were positively stained. By staining ultrathin sections with anti-CA IV/immunogold, CA IV could also be demonstrated at the latter two locations, including the specialized sarcolemmal structures intercalated discs, and T-tubules. In addition, by this method CA IV was seen to be associated with the sarcoplasmic reticulum (SR). The absence of immunostaining in SR and/or SL with some techniques probably indicates a problem of accessibility of the antigenic sites. In line with the immunohistochemical results, CA IV mRNA expression was visualized in both endothelial and muscle cells by in situ hybridization histochemistry. (J Histochem Cytochem 46:855–861, 1998)

Key Words: carbonic anhydrase IV, capillaries, sarcolemma, sarcoplasmic reticulum, heart, skeletal muscle, immunohistochemistry, in situ hybridization


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