Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lukas, J.–R.
Right arrow Articles by Mayr, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lukas, J.–R.
Right arrow Articles by Mayr, R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 46, 901-910, August 1998, Copyright © 1998, The Histochemical Society, Inc.

ARTICLE

Carbocyanine Postmortem Neuronal Tracing: Influence of Different Parameters on Tracing Distance and Combination with Immunocytochemistry

Julius–Robert Lukasa, Martin Aignerb, Michaela Denkb, Harald Heinzlc, Martin Buriand, and Robert Mayrb
a Department of Ophthalmology and Optometry, Medical School, University of Vienna, Vienna, Austria
b Institute of Anatomy, Department 2, Medical School, University of Vienna, Vienna, Austria
c Department of Medical Computer Sciences, Section of Clinical Biometrics, Medical School, University of Vienna, Vienna, Austria
d Department of Ear, Nose, and Throat, Medical School, University of Vienna, Vienna, Austria

Correspondence to: Julius–Robert Lukas, Institute of Anatomy, Dept. 2, Währingerstr. 13, A-1090 Vienna, Austria.

Carbocyanines (DiI, DiA, DiO) are able to travel along membranes by diffusion and therefore have been used as postmortem neuronal tracers in aldehyde-fixed tissues. Surprisingly, detailed data on the influence of different parameters on tracing distances are still missing. This study was carried out to optimize tracing procedures and to reveal the validity of the combination of postmortem tracing with immunocytochemistry. Carbocyanine crystals were applied to the cervical spinal cord, sciatic nerves, and brachial plexuses of humans and guinea pigs. Incubation in the dark at 37C for 12–15 weeks proved optimal to achieve longest tracing distances (28.9 ± 2.2 mm) in human and animal tissues. Longer incubation times and incubation temperatures higher than 37C did not result in longer tracing distances. No differences were evident between adult and newborn animals and between central and peripheral nervous system. The diffusion coefficient for DiI was calculated to be 2.5 x 10-7 cm2 sec-1. After application of DiI to nerves of guinea pig extraocular muscles, DiI-positive afferent perikarya were observed in the anteromedial part of the trigeminal ganglion. These perikarya were identified by calcitonin gene-related peptide immunoreactivity (CGRP-IR). The percentage of CGRP-IR neurons after tracing was concordant with the percentage of CGRP-IR in trigeminal ganglia exclusively processed for CGRP-IR without previous postmortem tracing. These results demonstrate carbocyanines to be specific tracers for exact neuronal mapping studies. (J Histochem Cytochem 46:901–910, 1998)

Key Words: carbocyanines, postmortem, neuronal tracing, CGRP, immunocytochemistry, double labeling, extraocular muscles, human, guinea pig


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
J. E. Murphy, R. S. Vohra, S. Dunn, Z. G. Holloway, A. P. Monaco, S. Homer-Vanniasinkam, J. H. Walker, and S. Ponnambalam
Oxidised LDL internalisation by the LOX-1 scavenger receptor is dependent on a novel cytoplasmic motif and is regulated by dynamin-2
J. Cell Sci., July 1, 2008; 121(13): 2136 - 2147.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
E. Tardif, A. Probst, and S. Clarke
Laminar Specificity of Intrinsic Connections in Broca's Area
Cereb Cortex, December 1, 2007; 17(12): 2949 - 2960.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
E. C. Tsai, R. L. van Bendegem, S. W. Hwang, and C. H. Tator
A Novel Method for Simultaneous Anterograde and Retrograde Labeling of Spinal Cord Motor Tracts in the Same Animal
J. Histochem. Cytochem., September 1, 2001; 49(9): 1111 - 1122.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
C. Shiba, T. Daikoku, F. Goshima, H. Takakuwa, Y. Yamauchi, O. Koiwai, and Y. Nishiyama
The UL34 gene product of herpes simplex virus type 2 is a tail-anchored type II membrane protein that is significant for virus envelopment
J. Gen. Virol., October 1, 2000; 81(10): 2397 - 2405.
[Abstract] [Full Text]

Home page
 IOVSHome page
J.-R. Lukas, R. Blumer, M. Denk, I. Baumgartner, W. Neuhuber, and R. Mayr
Innervated Myotendinous Cylinders in Human Extraocular Muscles
Invest. Ophthalmol. Vis. Sci., August 1, 2000; 41(9): 2422 - 2431.
[Abstract] [Full Text]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1998

 
Purchase HCS Short Course Manual on HCS site