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Journal of Histochemistry and Cytochemistry, Vol. 46, 963-970, August 1998, Copyright © 1998, The Histochemical Society, Inc.

ARTICLE

Translocation of 60S Ribosomal Subunit in Spreading Cardiac Myocytes

Terje H. Larsena and Thorvald Sætersdalb
a Experimental Cardiology Unit, Departments of Radiology, University of Bergen, Bergen, Norway
b Anatomy and Cell Biology, University of Bergen, Bergen, Norway

Correspondence to: Terje H. Larsen, Experimental Cardiology Unit, Dept. of Radiology, U. of Bergen, Årstadveien 19, N-5009 Bergen, Norway.

Cardiac myocytes in culture undergo considerable structural reorganization. The remodeling of the myofibrils and the nonmyofibrillar cytoskeleton that occurs in the spreading cardiac myocytes resembles the cellular features observed in the hypertrophying heart. In this study we examined the distribution of the large 60S ribosomal subunit in freshly isolated cardiac myocytes and during the course of attachment and spreading in culture. Initially, anti-60S immunolabeling was scattered widely throughout the sarcoplasm of the dissociated cardiac myocytes. After attachment to the substrate, the 60S ribosomal subunit attained wide sarcoplasmic localization before a sarcomere-related staining pattern appeared in the spreading cell. Double labeling experiments with {alpha}-actinin confirmed co-localization of the 60S ribosomal subunit with nascent and mature myofibrils. These findings demonstrate that translocation of the 60S ribosomal subunit coincides with the cytoskeletal reorganization taking place in these cells. Moreover, the close association between the myofibrils indicates a particular role for the ribosomes in maintenance and growth of the contractile apparatus. (J Histochem Cytochem 46:963–969, 1998)

Key Words: cardiac myocytes, ribosomes, 60S ribosomal subunit, immunofluorescence, microscopy, immunogold electron, microscopy, silver enhancement, myofibrillogenesis, hypertrophy


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