Journal of Histochemistry and Cytochemistry
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Journal of Histochemistry and Cytochemistry, Vol. 46, 1061-1072, September 1998, Copyright © 1998, The Histochemical Society, Inc.

ARTICLE

Expression of Gross Cystic Disease Fluid Protein-15/Prolactin-inducible Protein in Rat Salivary Glands

Lily Mirelsa, Arthur R. Handb, and Holly J. Braninc
a Department of Molecular and Cell Biology, University of California, Berkeley, California
b Department of Pediatric Dentistry, University of Connecticut Health Center, Farmington, Connecticut
c Biology Department, University of California, San Diego, California

Correspondence to: Lily Mirels, Dept. of Molecular and Cell Biology, Div. of Genetics, 401 Barker Hall #3204, University of California, Berkeley, CA 94720-3204..

Gross cystic disease fluid protein-15 (GCDFP-15)/prolactin-inducible protein (PIP) is present at moderate levels in human submandibular and sublingual glands and is barely detectable in human parotid gland. The rodent homologue, PIP, has previously been identified in adult submandibular and lacrimal glands. Here we present the molecular characterization of rat PIP and show that this protein is a product of neonatal and adult rat submandibular, sublingual, and parotid glands. cDNA clones encoding rat PIP were isolated and sequenced. The deduced amino acid sequence of rat PIP shows 56% overall identity and 80% similarity with mouse PIP. By SDS-PAGE, secreted rat PIP has an apparent Mr of 17,000, with a minor proportion present as Mr 20–22,000 N-glycosylated forms. PIP was localized in rat salivary glands by immunogold silver staining. PIP was identified in acinar cells of developing and mature submandibular and parotid glands and at very low levels in sublingual gland serous demilunes. Typically, rat submandibular gland secretory proteins are produced by either acinar cell progenitors (Type III cells) or mature acinar cells. The expression pattern observed for PIP is similar to that previously reported for salivary peroxidase, an important component of nonimmune mucosal defense.

(J Histochem Cytochem 46:1061–1071, 1998)

Key Words: submandibular gland, sublingual gland, parotid gland, prolactin-inducible protein, GCDFP-15, rat, development


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