Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Biggiogera, M.
Right arrow Articles by Pellicciari, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Biggiogera, M.
Right arrow Articles by Pellicciari, C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 46, 999-1006, September 1998, Copyright © 1998, The Histochemical Society, Inc.

ARTICLE

Nuclear RNA Is Extruded from Apoptotic Cells

Marco Biggiogeraa, Maria Grazia Bottonea, and Carlo Pellicciaria
a Dipartimento di Biologia Animale, Laboratorio di Istologia, and Centro di Studio per l'Istochimica del CNR, University of Pavia, Pavia, Italy

Correspondence to: Marco Biggiogera, Dipartimento di Biologia Animale, Laboratorio di Istologia, U. of Pavia, Piazza Botta 10, 27100 Pavia, Italy..

During spontaneous apoptosis of thymocytes there is extrusion of ribonucleoproteins (RNPs) from the cell. The aim of this investigation was to elucidate whether the RNP aggregates in apoptotic cells and bodies still contain RNA in an appreciable amount. We demonstrated by specific cytochemical techniques that the aggregates of nuclear RNPs extruded in the cytoplasm of spontaneously apoptotic thymocytes contain RNA in a sufficient amount to be detected cytochemically. These heterogeneous ectopic RNP-derived structures (HERDS) are formed by perichromatin fibrils, interchromatin granules, perichromatin granules, and nucleolar material. The RNA detected inside these clusters should therefore correspond to both mRNA and snRNA as well as to rRNA. We never observed DNA-contaning aggregates in the cytoplasm of apoptotic thymocytes. The presence of RNA in the HERDS that may be released from apoptotic cells suggests that the decrease in the amount of total RNA during apoptosis may be mostly linked to cellular extrusion rather than to degradation of RNA by RNase activities. Another interesting aspect of these results lies in the hypothesis of apoptosis as a possible cause for the presence of autoantibodies in the serum of patients with systemic autoimmune diseases. (J Histochem Cytochem 46:999–1005, 1998)

Key Words: apoptosis, RNA, ribonucleoproteins, thymocytes, osmium ammine, terbium, cytochemistry


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
C. D. Sadik, M. Bachmann, J. Pfeilschifter, and H. Muhl
Activation of interferon regulatory factor-3 via toll-like receptor 3 and immunomodulatory functions detected in A549 lung epithelial cells exposed to misplaced U1-snRNA
Nucleic Acids Res., June 18, 2009; (2009) gkp525v1.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
Y. Li, D. Elashoff, M. Oh, U. Sinha, M. A.R. St John, X. Zhou, E. Abemayor, and D. T. Wong
Serum Circulating Human mRNA Profiling and Its Utility for Oral Cancer Detection
J. Clin. Oncol., April 10, 2006; 24(11): 1754 - 1760.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
T. El-Hefnawy, S. Raja, L. Kelly, W. L. Bigbee, J. M. Kirkwood, J. D. Luketich, and T. E. Godfrey
Characterization of Amplifiable, Circulating RNA in Plasma and Its Potential as a Tool for Cancer Diagnostics
Clin. Chem., March 1, 2004; 50(3): 564 - 573.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. A. Gebska, I. Titley, H. F. Paterson, R. M. Morilla, D. C. Davies, A. M. Gruszka-Westwood, V. V. Kakkar, S. Eccles, and M. F. Scully
High-affinity binding sites for heparin generated on leukocytes during apoptosis arise from nuclear structures segregated during cell death
Blood, March 15, 2002; 99(6): 2221 - 2227.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
M. BIGGIOGERA and C. PELLICCIARI
Heterogeneous ectopic RNP-derived structures (HERDS) are markers of transcriptional arrest
FASEB J, April 1, 2000; 14(5): 828 - 834.
[Abstract] [Full Text]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1998

 
Purchase HCS Short Course Manual on HCS site