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Journal of Histochemistry and Cytochemistry, Vol. 47, 113-118, January 1999, Copyright © 1999, The Histochemical Society, Inc.

ARTICLE

Malignancies of the Uterine Corpus and Immunoreactivity Score of the DNA "Mismatch-Repair" Enzyme Human Mut-S-Homologon-2

M. Friedricha, C. Villena–Heinsena, K. Reitnauerb, W. Schmidta, W. Tilgenc, and J. Reichrathc
a Departments of Obstetrics and Gynecology, Universität des Saarlandes, Homburg/Saar, Germany
b Pathology, Universität des Saarlandes, Homburg/Saar, Germany
c Dermatology, Universität des Saarlandes, Homburg/Saar, Germany

Correspondence to: M. Friedrich, Universitäts-Frauenklinik, Gebäude 9, D-66421 Homburg/Saar, Germany..

We analyzed human Mut-S-Homologon-2 expression in normal endometrial tissue (n = 15) and malignancies of the uterine corpus (n = 40). Human Mut-S-Homologon-2 protein was investigated immunohistochemically on frozen sections, using a highly sensitive streptavidin–peroxidase technique and a specific mouse monoclonal antibody (clone FE11). Human Mut-S-Homologon-2 labeling pattern was compared with the staining pattern of the proliferation marker Ki-67 in the same tumors. A human Mut-S-Homologon-2 immunoreactivity score (human Mut-S-Homologon-2-IRS: negative 0–1; weak 2–3; moderate 4–6; strong 8–12) for semiquantitative analysis of human Mut-S-Homologon-2 expression is presented. In normal endometrial tissue samples we found weak nuclear immunoreactivity for human Mut-S-Homologon-2 in 67%, whereas the remaining 33% were negative for human Mut-S-Homologon-2 (mean human Mut-S-Homologon-2-IRS 1.25 ± 1.29). All malignancies of the uterine corpus analyzed revealed moderate to strong nuclear immunoreactivity (mean human Mut-S-Homologon-2-IRS 9.00, ± 3.16). Human Mut-S-Homologon-2 staining was heterogeneous, with visual differences among individual tumor cells. Expression of human Mut-S-Homologon-2 protein was consistently and strongly upregulated in tumor cells of malignancies of the uterine corpus compared with normal endometrial tissue (human Mut-S-Homologon-2-PP p<0.001; human Mut-S-Homologon-2-IS p<0.001; human Mut-S-Homologon-2-IRS p<0.001). No statistically significant correlation in comparing the labeling patterns for human Mut-S-Homologon-2 with the labeling patterns for Ki-67 (mean percentage of Ki-67-positive tumor cells 22.00% ± 17.20) was observed in malignancies of the uterine corpus (human Mut-S-Homologon-2-PP p=0.443; human Mut-S-Homologon-2-IS p=0.234; human Mut-S-Homologon-2-IRS p=0.173). Our findings indicate that human Mut-S-Homologon-2 is expressed in normal human endometrial tissue and that expression of human Mut-S-Homologon-2 may be of importance for the genetic stability of malignancies of the uterine corpus in vivo. (J Histochem Cytochem 47:113–118, 1999).

Key Words: human Mut-S-Homologon-2, DNA repair, malignancies of the uterine, corpus, carcinogenesis, immunohistochemistry


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