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Journal of Histochemistry and Cytochemistry, Vol. 47, 91-98, January 1999, Copyright © 1999, The Histochemical Society, Inc.

ARTICLE

Exogenous 17ß-Estradiol Blocks Alpha and Mu but Not Pi Class Glutathione S-Transferase Immunoreactivity in Epithelium of Syrian Hamster Vas Deferens

Chad E. Hudsona, John E. DeHavena, Bradley A. Schulteb, and James S. Norrisa
a Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, South Carolina
b Departments of Pathology and Laboratory Medicine and Otolaryngology and Communicative Sciences, Medical University of South Carolina, Charleston, South Carolina

Correspondence to: James S. Norris, Dept. of Microbiology and Immunology, Medical Univ. of South Carolina, 173 Ashley Ave., Charleston, SC 29425-2230..

Members of the glutathione S-transferase (GST) family of detoxification enzymes play a role in chemotherapy resistance in certain cancers but have not been directly implicated as agents whose absence may predispose tissues to hormonally induced tumorigenesis. Here we report the development of a polyclonal antiserum to a hamster mu class GST, and immunohistochemical analysis of alpha, mu, and pi class GSTs to study the effects of hormone treatment on their expression in reproductive tract tissues of male golden Syrian hamsters. These animals develop leiomyosarcomas in the vas deferens after treatment with testosterone propionate (TP) and 17ß-estradiol (E2). High levels of all three GST classes were detected throughout the reproductive tract epithelium of control animals. In 100% of the experimental animals, 4 weeks of treatment either with E2 alone, or with E2 plus TP promoted a complete loss of immunostaining for alpha and mu class GSTs, but not for pi class GSTs, only in the epithelial lining of the vas deferens. In contrast, treatment with TP alone resulted in moderate hyperplasia of smooth muscle in the proximal vas deferens, with no cellular atypia and no changes in immunoreactivity of any of the GST classes. The consistent and site-specific nature of these results strongly suggests a functional role for GSTs in hormonally induced carcinogenic process. (J Histochem Cytochem 47:91–98, 1999)

Key Words: glutathione S-transferase, hormonal carcinogenesis, 17ß-estradiol


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