Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Skinner, M. A.
Right arrow Articles by Wildeman, A. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Skinner, M. A.
Right arrow Articles by Wildeman, A. G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 47, 1247-1254, October 1999, Copyright © 1999, The Histochemical Society, Inc.

ARTICLE

Stage-dependent Redistribution of the V-ATPase During Bovine Implantation

Mhairi A. Skinnera, Leslie A. MacLarenb, and Alan G. Wildemana
a Department of Molecular Biology and Genetics, University of Guelph, Guelph, Ontario, Canada
b Animal Science Department, Nova Scotia Agricultural College, Truro, Nova Scotia, Canada

Correspondence to: Alan G. Wildeman, Dept. of Molecular Biology and Genetics, Univ. of Guelph, Guelph, Ontario, Canada N1G 2W1.

The 16-kD subunit of the vacuolar H+-ATPase (V-ATPase), or ductin, is essential for the activity of this proton pump and has roles in intercellular communication and control of cell growth and differentiation. The V-ATPase is important for acidification-dependent degradation of tissue matrices through which some cell types move, and for pH regulation across some epithelial cell layers. Placentation involves intricate signaling, cell proliferation, and controlled invasion. We examined the distribution of three subunits of the V-ATPase in bovine trophoblast and endometrium at the time of implantation to determine the relationship of ductin expression to that of two other subunits, A (approximately 73 kD) and B (approximately 58 kD). Epithelial expression of all three subunits was observed, and in nonpregnant animals this expression was apical. As pregnancy proceeded, expression of all subunits became pericellular in luminal but not glandular epithelium, suggesting a redistribution of V-ATPase activity. The trophoblast expressed all three subunits during initial contact with the epithelium. In the stroma, ductin expression was reduced after implantation, and we discuss the possibility that ductin plays a role in the shifting communication between stromal and epithelial cells induced by embryo attachment. (J Histochem Cytochem 47:1247–1254, 1999)

Key Words: implantation, V-ATPase, ductin, bovine, endometrium


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S.-H. Chen, M. R. Bubb, E. G. Yarmola, J. Zuo, J. Jiang, B. S. Lee, M. Lu, S. L. Gluck, I. R. Hurst, and L. S. Holliday
Vacuolar H+-ATPase Binding to Microfilaments: REGULATION IN RESPONSE TO PHOSPHATIDYLINOSITOL 3-KINASE ACTIVITY AND DETAILED CHARACTERIZATION OF THE ACTIN-BINDING SITE IN SUBUNIT B
J. Biol. Chem., February 27, 2004; 279(9): 7988 - 7998.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
H. Tinel, H.-W. Denker, and M. Thie
Calcium influx in human uterine epithelial RL95-2 cells triggers adhesiveness for trophoblast-like cells. Model studies on signalling events during embryo implantation
Mol. Hum. Reprod., December 1, 2000; 6(12): 1119 - 1130.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. S. Holliday, M. Lu, B. S. Lee, R. D. Nelson, S. Solivan, L. Zhang, and S. L. Gluck
The Amino-terminal Domain of the B Subunit of Vacuolar H+-ATPase Contains a Filamentous Actin Binding Site
J. Biol. Chem., October 6, 2000; 275(41): 32331 - 32337.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1999