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Journal of Histochemistry and Cytochemistry, Vol. 47, 1405-1416, November 1999, Copyright © 1999, The Histochemical Society, Inc.

ARTICLE

Quantitative Comparison of Growth-associated Protein GAP-43, Neuron-specific Enolase, and Protein Gene Product 9.5 as Neuronal Markers in Mature Human Intestine

Pälvi Ventoa and Seppo Soinilab
a Second Department of Surgery, Helsinki University Central Hospital, Helsinki, Finland
b Department of Neurology and Institute of Biomedicine, University of Helsinki, Helsinki, Finland

Correspondence to: Pälvi Vento, Second Dept. of Surgery, Helsinki U. Central Hospital, Haartmaninkatu 4, 00290 Helsinki, Finland.

This study was performed to compare GAP-43, PGP 9.5, synaptophysin, and NSE as neuronal markers in the human intestine. GAP-43-immunoreactive nerve fibers were abundant in all layers of the ileum and colon. GAP-43 partially co-localized partially with every neuropeptide (VIP, substance P, galanin, enkephalin) studied. All neuropeptide-immunoreactive fibers also showed GAP-43 reactivity. By blind visual estimation, the numbers of GAP-43-immunoreactive fibers in the lamina propria were greater than those of PGP 9.5, synaptophysin, or NSE. In the muscle layer, visual estimation indicated that the density of GAP-43-immunoreactive fiber profiles was slightly greater than that of the others. The number and intensity of GAP-43-, PGP 9.5-, and NSE-immunoreactive fibers were estimated in sections of normal human colon and ileum using computerized morphometry. In the colon, the numbers of GAP-43-immunoreactive nerve profiles per unit area and their size and intensity were significantly greater than the values for PGP and NSE. A similar trend was observed in the ileum. Neuronal somata lacked or showed only weak GAP-43 immunoreactivity, variable PGP 9.5 immunoreactivity, no synaptophysin immunoreactivity, and moderate to strong NSE immunoreactivity. We conclude that GAP-43 is the superior marker of nerve fibers in the human intestine, whereas NSE is the marker of choice for neuronal somata. (J Histochem Cytochem 47:1405–1415, 1999)

Key Words: GAP-43, PGP 9.5, neuron-specific enolase, synaptophysin, enteric nervous system, immunohistochemistry, morphometry, neuropeptide


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P. Vento, T. Kiviluoto, U. Keränen, H. J. Järvinen, E. Kivilaakso, and S. Soinila
Quantitative Comparison of Growth-associated Protein-43 and Substance P in Ulcerative Colitis
J. Histochem. Cytochem., June 1, 2001; 49(6): 749 - 758.
[Abstract] [Full Text]



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