Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kavanagh, E.
Right arrow Articles by Ashhurst, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kavanagh, E.
Right arrow Articles by Ashhurst, D. E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 47, 1603-1616, December 1999, Copyright © 1999, The Histochemical Society, Inc.

ARTICLE

Development and Aging of the Articular Cartilage of the Rabbit Knee Joint: Distribution of Biglycan, Decorin, and Matrilin-1

Emma Kavanagha and Doreen E. Ashhursta
a Department of Anatomy, St George's Hospital Medical School, London, United Kingdom

Correspondence to: Doreen E. Ashhurst, Dept. of Anatomy, St George's Hospital Medical School, Cramner Terrace, Tooting, London SW17 ORE, UK.

We determined the distributions of the small proteoglycans biglycan and decorin and the glycoprotein matrilin-1 (cartilage matrix protein) during development and aging of articular cartilage in the rabbit knee joint. Before cavitation, the matrices of the interzone and the adjacent epiphyseal cartilage do not contain biglycan or decorin, but some chondrocytes express their mRNAs. Matrilin-1 is found only in the deeper epiphyseal cartilage. After cavitation, biglycan and decorin are detected in the presumptive articular cartilage, but there is no matrilin-1. All are present in the underlying epiphyseal cartilage. In the neonate, the epiphyseal cartilage is ossified and the articular cartilage becomes a discrete layer. Biglycan and decorin accumulate in the articular cartilage, but matrilin-1 remains confined to the residual epiphyseal cartilage. In the adult, the distributions of biglycan and decorin are highly variable. Decorin tends to be confined to the central region; matrilin-1 is absent. The findings indicate that the articular and epiphyseal cartilages are different from the earliest developmental stages. The epiphyseal cartilage can be identified by its possession of matrilin-1. Epiphyseal cartilage is removed during development to leave the articular cartilage. The relationships between the distributions of decorin and matrilin-1 and the fibrillar collagens are discussed. (J Histochem Cytochem 47:1603–1615, 1999)

Key Words: articular cartilage, biglycan, decorin, matrilin-1, development, aging, knee joint, mRNA, in situ hybridization, immunohistochemistry


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
D. R. Studelska, L. Mandik-Nayak, X. Zhou, J. Pan, P. Weiser, L. M. McDowell, H. Lu, H. Liapis, P. M. Allen, F. F. Shih, et al.
High Affinity Glycosaminoglycan and Autoantigen Interaction Explains Joint Specificity in a Mouse Model of Rheumatoid Arthritis
J. Biol. Chem., January 23, 2009; 284(4): 2354 - 2362.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
E. Kavanagh, A. C. Osborne, D. E. Ashhurst, and A. A. Pitsillides
Keratan Sulfate Epitopes Exhibit a Conserved Distribution During Joint Development That Remains Undisclosed on the Basis of Glycosaminoglycan Charge Density
J. Histochem. Cytochem., August 1, 2002; 50(8): 1039 - 1047.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
E. Kavanagh and D. E. Ashhurst
Distribution of Biglycan and Decorin in Collateral and Cruciate Ligaments and Menisci of the Rabbit Knee Joint
J. Histochem. Cytochem., July 1, 2001; 49(7): 877 - 886.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1999

 
Purchase HCS Short Course Manual on HCS site