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Journal of Histochemistry and Cytochemistry, Vol. 47, 1625-1632, December 1999, Copyright © 1999, The Histochemical Society, Inc.

ARTICLE

Immunohistochemical Distribution of Inter-{alpha}-trypsin Inhibitor Chains in Normal and Malignant Human Lung Tissue

Jeannette Bourguignona, Hélène Borghia, Richard Sesboüéa, Maryam Diarra–Mehrpoura, Jean-François Bernaudinc, Josette Métayerb, Jean-Pierre Martina, and Luc Thibervillea
a INSERM Unité 295, Faculté de Médecine-Pharmacie, Rouen, France
b Service d'Anatomie et Cytologie Pathologiques, Centre Hospitalier Universitaire, Rouen, France
c Laboratoire de Biologie Tumorale, Hôpital Tenon, Paris, France

Correspondence to: Jeannette Bourguignon, INSERM, Unité 295, Faculté de Médecine-Pharmacie de Rouen, 22 Boulevard Gambetta, F-76183 Rouen Cedex, France.

The inter-{alpha}-trypsin inhibitor (ITI) family is a group of plasma proteins built up from heavy (HC1, HC2, HC3) and light (bikunin) chains synthesized in the liver. In this study we determined the distribution of ITI constitutive chains in normal and cancerous lung tissues using polyclonal antibodies. In normal lung tissue, H2, H3, and bikunin chains were found in polymorphonuclear cells, whereas H1 and bikunin proteins were found in mast cells. Bikunin was further observed in bronchoepithelial mucous cells. In lung carcinoma, similar findings were obtained on infiltrating polymorphonuclear and mast cells surrounding the tumor islets. Highly differentiated cancerous cells displayed strong intracytoplasmic staining with H1 and bikunin antiserum in both adenocarcinoma and squamous cell carcinoma. Moreover, weak but frequent H2 expression was observed in adenocarcinoma cells, whereas no H3-related protein could be detected in cancer cells. Local lung ITI expression was confirmed by RT-PCR. Although the respective role of inflammatory and tumor cells in ITI chain synthesis cannot be presently clarified, these results show that heavy chains as well as bikunin are involved in malignant transformation of lung tissue. (J Histochem Cytochem 47:1625–1632, 1999)

Key Words: intra-{alpha}-trypsin inhibitor, ITI heavy chains, bikunin, immunohistochemistry, RT-PCR, normal lung tissue, lung adenocarcinoma, squamous cell lung carcinoma


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