Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dobbs, L. G.
Right arrow Articles by Froh, D. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dobbs, L. G.
Right arrow Articles by Froh, D. K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 47, 129-138, February 1999, Copyright © 1999, The Histochemical Society, Inc.

ARTICLE

HTI56, an Integral Membrane Protein Specific to Human Alveolar Type I Cells

Leland G. Dobbsa, Robert F. Gonzaleza, Lennell Allena, and Deborah K. Froha
a Departments of Medicine and Pediatrics and the Cardiovascular Research Institute, University of California San Francisco, San Francisco, California

Correspondence to: Leland G. Dobbs, U. of California San Francisco: Laurel Heights, 3333 California St., Suite 150, Box 1245, San Francisco, CA 94118.

The alveolar epithelium is composed of two morphologically distinct types of cells, Type I and Type II cells. The thin cytoplasmic extensions of Type I cells cover more than 95% of the internal surface area of the lungs. Type I cells provide the very short diffusion pathway essential for gas exchange. Because there were no biochemical markers specific for human Type I cells, we developed a strategy to produce a monoclonal antibody (MAb) specific for human Type I cells. Isolated human lung cells were used as immunogens; >5000 clones from seven fusions were screened to identify an MAb specific for a 56-kD protein of Type I cells, HTI56. By Western blotting, HTI56 is unique to the lung. By immunoelectron microscopy, it is localized to the Type I cell apical plasma membrane. The pI of HTI56 is 2.5–3.5. HTI56 is glycosylated and has the biochemical characteristics of an integral membrane protein. HTI56 is detectable by Week 20 of gestation and its expression increases in fetal lung explant culture. HTI56 should be useful as a marker for human Type I cells both morphologically and biochemically. It may also be useful in studies of disease and as a marker for lung injury. (J Histochem Cytochem 47:129–137, 1999)

Key Words: HTI56, type I cells, pulmonary, epithelium, lung, lung injury, integral membrane protein


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Respir. Crit. Care Med.Home page
T. Uchida, M. Shirasawa, L. B. Ware, K. Kojima, Y. Hata, K. Makita, G. Mednick, Z. A. Matthay, and M. A. Matthay
Receptor for Advanced Glycation End-Products Is a Marker of Type I Cell Injury in Acute Lung Injury
Am. J. Respir. Crit. Care Med., May 1, 2006; 173(9): 1008 - 1015.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. D. Johnson, H.-F. Bao, M. N. Helms, X.-J. Chen, Z. Tigue, L. Jain, L. G. Dobbs, and D. C. Eaton
Functional ion channels in pulmonary alveolar type I cells support a role for type I cells in lung ion transport
PNAS, March 28, 2006; 103(13): 4964 - 4969.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. Gonzalez, Y. H. Yang, C. Griffin, L. Allen, Z. Tigue, and L. Dobbs
Freshly isolated rat alveolar type I cells, type II cells, and cultured type II cells have distinct molecular phenotypes
Am J Physiol Lung Cell Mol Physiol, January 1, 2005; 288(1): L179 - L189.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
M.C. McElroy and M. Kasper
The use of alveolar epithelial type I cell-selective markers to investigate lung injury and repair
Eur. Respir. J., October 1, 2004; 24(4): 664 - 673.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
K. Dahlin, E. M. Mager, L. Allen, Z. Tigue, L. Goodglick, M. Wadehra, and L. Dobbs
Identification of Genes Differentially Expressed in Rat Alveolar Type I Cells
Am. J. Respir. Cell Mol. Biol., September 1, 2004; 31(3): 309 - 316.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
M. Shirasawa, N. Fujiwara, S. Hirabayashi, H. Ohno, J. Iida, K. Makita, and Y. Hata
Receptor for advanced glycation end-products is a marker of type I lung alveolar cells
Genes Cells, February 1, 2004; 9(2): 165 - 174.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
V. NEWMAN, R. F. GONZALEZ, M. A. MATTHAY, and L. G. DOBBS
A Novel Alveolar Type I Cell-Specific Biochemical Marker of Human Acute Lung Injury
Am. J. Respir. Crit. Care Med., March 1, 2000; 161(3): 990 - 995.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
T.-H. Chang and E. Szabo
Induction of Differentiation and Apoptosis by Ligands of Peroxisome Proliferator-activated Receptor {{gamma}} in Non-Small Cell Lung Cancer
Cancer Res., February 1, 2000; 60(4): 1129 - 1138.
[Abstract] [Full Text]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1999