Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Romero, M. I.
Right arrow Articles by Smith, G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Romero, M. I.
Right arrow Articles by Smith, G. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 47, 265-272, February 1999, Copyright © 1999, The Histochemical Society, Inc.

TECHNICAL NOTE

Visualization of Axonally Transported Horseradish Peroxidase Using Enhanced Immunocytochemical Detection: A Direct Comparison with the Tetramethylbenzidine Method

Mario I. Romeroa, Martha A. Romeroa, and George M. Smitha
a Department of Anesthesiology and Pain Management, University of Texas Southwestern Medical Center, Dallas, Texas

Correspondence to: Mario I. Romero, Dept. of Anesthesiology and Pain Management, U. of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75235.

Visualization of the neuronal tract tracer horseradish peroxidase (HRP) is commonly achieved through the histochemical detection of its enzymatic activity using 3,3',5,5'-tetramethylbenzidine (TMB) as a chromogen. However, the TMB product is unstable and is incompatible with tissue processing methods that render the enzyme inactive, or when a combination of HRP tract tracing with neuronal phenotype identification is required. In this study we evaluated the applicability of the immunocytochemical detection method for horseradish peroxidase (HRP) visualization using an enhanced detection meth-od based on the Elite ABC peroxidase amplification protocol. The results provide evidence for the immunocytochemical visualization of both anterograde and transganglionic HRP transport in the rat spinal cord. This immunocytochemical method not only showed similar sensitivity to the TMB protocol in detecting HRP-labeled motor neuron perikarya but provided enhanced resolution in the identification of individual neuronal fibers compared to the TMB method. Immunodetection of the HRP tracer also allowed its co-localization with specific neuronal markers using double immunofluorescence techniques. These results offer the first demonstration that sensitive identification of axonally transported HRP can be achieved by immunocytochemistry and provides further support for its use in HRP tract tracing studies. (J Histochem Cytochem 47:265–272, 1999)

Key Words: neuroanatomic tract tracing methods, horseradish peroxidase, tetramethylbenzidine, Elite ABC, immunocytochemistry, double labeling, spinal cord


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
E. C. Tsai, R. L. van Bendegem, S. W. Hwang, and C. H. Tator
A Novel Method for Simultaneous Anterograde and Retrograde Labeling of Spinal Cord Motor Tracts in the Same Animal
J. Histochem. Cytochem., September 1, 2001; 49(9): 1111 - 1122.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1999

 
Purchase HCS Short Course Manual on HCS site