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Journal of Histochemistry and Cytochemistry, Vol. 47, 289-302, March 1999, Copyright © 1999, The Histochemical Society, Inc.

ARTICLE

Cellular and Subcellular Expression of Golf/Gs and Gq/G11 {alpha}-Subunits in Rat Pancreatic Endocrine Cells

Any Astesanoa, Karine Regnaulda, Nathalie Ferranda, Diane Gingrasb, Moïse Bendayanb, Gabriel Rosselina, and Shahin Emamia
a Unité de Recherches sur la Signalisation et les Fonctions Cellulaires: Applications au Diabète et aux Cancers Digestifs, Institut National de la Santé et de la Recherche Médicale U482, Centre de Recherches Paris-Saint-Antoine, Paris, France
b Départment de Pathologie et de Biologie Cellulaire, Université de Montréal, Montréal, Quebec, Canada

Correspondence to: Any Astesano, Unité de Recherches sur la Signalisation et les Fonctions Cellulaires: Applications au Diabète et aux Cancers Digestifs, Inst. National de la Santé et de la Recherche Médicale U482, Centre de Recherches Paris-St-Antoine, 184 rue du Fbg. St Antoine, 75571 Paris Cedex 12, France.

We studied the cellular and subcellular localization of G{alpha}-subunits in pancreas by immunocytochemistry. Golf{alpha} and G11{alpha} were specifically localized in islet insulin B-cells and glucagon A-cells, respectively. Gs{alpha} and Gq{alpha} labeling was more abundant in B-cells. The presence of Golf{alpha} in B-cells was confirmed by in situ hybridization. In B-cells, Golf{alpha} and Gs{alpha} were found in the Golgi apparatus, plasma membrane (PM) and, remarkably, in mature and immature insulin secretory granules, mainly at the periphery of the insulin grains. Gq{alpha} was detected on the rough endoplasmic reticulum (RER) near the Golgi apparatus. In A-cells, the G{alpha}-subunits were mostly within the glucagon granules: G11{alpha} gave the strongest signal, Gs{alpha} less strong, Gq was scarce, and Golf was practically absent. Gq{alpha} and Gs{alpha} immunoreactivity was detected in acinar cells, although it was much weaker than that in islet cells. The cell-dependent distribution of the G{alpha}-subunits indicates that the stimulatory pathways for pancreatic function differ in acinar and in islet B- and A-cells. Furthermore, the G-protein subunits in islet cell secretory granules might be functional and participate in granule trafficking and hormone secretion. (J Histochem Cytochem 47:289–302,1999)

Key Words: G proteins, Golf/s{alpha}, Gq/11{alpha}, signal transduction, diabetes, islets of Langerhans, B-cell, immunocytochemistry, confocal microscopy, in situ hybridization, electron microscopy


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