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Journal of Histochemistry and Cytochemistry, Vol. 47, 343-352, March 1999, Copyright © 1999, The Histochemical Society, Inc.

ARTICLE

Unique Localization of Protein Gene Product 9.5 in Type B Synoviocytes in the Joints of the Horse

Hiroko P. Kitamuraa, Haruko Yanasea, Hiroshi Kitamurab, and Toshihiko Iwanagaa
a Laboratories of Anatomy, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan
b Biochemistry, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan

Correspondence to: Toshihiko Iwanaga, Lab. of Anatomy, Graduate School of Veterinary Medicine, Hokkaido Univ., Kita-18 Nishi-9, Kita-ku, Sapporo 060-0818, Japan.

Fibroblast-like (Type B) synoviocytes are cells in the synovial membrane that are responsible for production of both synovial fluid and the extracellular matrix in the synovial intima. Immunostaining of the horse synovial membrane for protein gene product (PGP) 9.5, which is a neuron-specific ubiquitin C-terminal hydrolase, demonstrated selective localization of the immunoreactivity in a synoviocyte population different from acid phosphatase-positive Type A synoviocytes. The immunoreactive cells were lined up in the synovial intima and extended dendritic processes towards the joint cavity to form a dense plexus on the surface. Electron microscopic examination clearly identified the PGP 9.5-immunoreactive cells as Type B synoviocytes characterized by developed rough endoplasmic reticulum and free ribosomes. Immunoreactivity for PGP 9.5 was diffusely distributed throughout the cytoplasm, including the tips of fine processes. Western and Northern blot analyses could not distinguish the corresponding protein and mRNA obtained from the brain and synovial membrane. The existence of the neuron-specific PGP 9.5 in Type B synoviocytes suggests a common mechanism regulating the protein metabolism between neurons and synoviocytes, and also provides a new cytochemical marker for identification of the cells. (J Histochem Cytochem 47:343–351, 1999)

Key Words: joint, protein gene product 9.5, synovial membrane, immunohistochemistry, horse


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