Journal of Histochemistry and Cytochemistry
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baskin, D. G.
Right arrow Articles by Kochan, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baskin, D. G.
Right arrow Articles by Kochan, J. P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 47, 353-362, March 1999, Copyright © 1999, The Histochemical Society, Inc.

ARTICLE

Leptin Receptor Long-form Splice-variant Protein Expression in Neuron Cell Bodies of the Brain and Co-localization with Neuropeptide Y mRNA in the Arcuate Nucleus

Denis G. Baskina,b,c, Michael W. Schwartza,b, Randy J. Seeleyd, Stephen C. Woodsd, Daniel Porte, Jr.a,b, John F. Breiningerb, Zoë Jonakb, Jonathan Schaeferb, Michael Krousea, Charles Burghardte, L. Arthur Campfielde, Paul Burne, and Jarema P. Kochane
a Veterans Affairs Puget Sound Health Care System, Seattle, Washington
b Departments of Medicine, University of Washington, Seattle, Washington
c Biological Structure, University of Washington, Seattle, Washington
d Psychology, University of Washington, Seattle, Washington
e Department of Metabolic Diseases, Hoffmann–La Roche, Inc., Nutley, New Jersey

Correspondence to: Denis G. Baskin, Div. of Endocrinology and Metabolism, Research Service, Mail Stop 151, Veterans Affairs Puget Sound Health Care System, 1660 S. Columbian Way, Seattle, WA 98108. E-mail: baskindg@u.washington.edu

Reduced leptin (Ob protein) signaling is proposed to be a stimulus for the activation of neuropeptide Y (NPY) gene activity and increased expression of mRNA for the long form of the leptin receptor (Ob-Rb) in the hypothalamic arcuate nucleus. To determine if Ob-Rb protein is expressed in arcuate nucleus NPY neurons, we developed an affinity-purified polyclonal antibody against amino acids 956–1102 of human Ob-Rb. This antibody specifically recognizes the cytoplasmic tail of Ob-Rb and does not react with shorter leptin-receptor variants. Western immunoblots of Ob-Rb-transfected COS cells showed a single 150-kD band, and immunofluorescence revealed intense perinuclear staining in the cytoplasm. A 150-kD band was also present in Western immunoblots of hypothalamus. Immunocytochemical staining of brain slices revealed immunoreactive Ob-Rb protein concentrated in many neuronal cell bodies in the same regions of the forebrain that also express Ob-Rb mRNA. In the hypothalamus, Ob-Rb-positive cell bodies were abundant in the arcuate nucleus and ventromedial nucleus, with lesser numbers in the dorsomedial nucleus and paraventricular nucleus. Immunostaining was also detected in cell bodies of pyramidal cell neurons of the pyriform cortex and cerebral cortex, in neurons of the thalamus, and on the surface of ependymal cells lining the third ventricle. The choroid plexus, which expresses the short Ob-Ra form, was negative. Combined immunocytochemistry for Ob-Rb protein and fluorescence in situ hybridization for NPY mRNA identified arcuate nucleus neurons containing both NPY mRNA and Ob-Rb protein. The present finding of Ob-Rb protein in neurons that express NPY mRNA supports the hypothesis that arcuate nucleus NPY neurons are direct targets of leptin and play an important role in regulation of food intake and body weight. (J Histochem Cytochem 47:353–362, 1999)

Key Words: obesity, food intake, body weight, neuropeptide Y, arcuate nucleus, hypothalamus


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. W. Hill, J. K. Elmquist, and C. F. Elias
Hypothalamic pathways linking energy balance and reproduction
Am J Physiol Endocrinol Metab, May 1, 2008; 294(5): E827 - E832.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Couturier, C. Sarkis, K. Seron, S. Belouzard, P. Chen, A. Lenain, L. Corset, J. Dam, V. Vauthier, A. Dubart, et al.
Silencing of OB-RGRP in mouse hypothalamic arcuate nucleus increases leptin receptor signaling and prevents diet-induced obesity
PNAS, December 4, 2007; 104(49): 19476 - 19481.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A.-S. Carlo, M. Pyrski, C. Loudes, A. Faivre-Baumann, J. Epelbaum, L. M. Williams, and W. Meyerhof
Leptin Sensitivity in the Developing Rat Hypothalamus
Endocrinology, December 1, 2007; 148(12): 6073 - 6082.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
M. M. Kamiji and A. Inui
Neuropeptide Y Receptor Selective Ligands in the Treatment of Obesity
Endocr. Rev., October 1, 2007; 28(6): 664 - 684.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
L. Shen, P. Tso, S. C. Woods, R. R. Sakai, W. S. Davidson, and M. Liu
Hypothalamic Apolipoprotein A-IV Is Regulated by Leptin
Endocrinology, June 1, 2007; 148(6): 2681 - 2689.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. Munzberg, E. E. Jobst, S. H. Bates, J. Jones, E. Villanueva, R. Leshan, M. Bjornholm, J. Elmquist, M. Sleeman, M. A. Cowley, et al.
Appropriate Inhibition of Orexigenic Hypothalamic Arcuate Nucleus Neurons Independently of Leptin Receptor/STAT3 Signaling
J. Neurosci., January 3, 2007; 27(1): 69 - 74.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. O. Huising, E. J. W. Geven, C. P. Kruiswijk, S. B. Nabuurs, E. H. Stolte, F. A. T. Spanings, B. M. L. Verburg-van Kemenade, and G. Flik
Increased Leptin Expression in Common Carp (Cyprinus carpio) after Food Intake But Not after Fasting or Feeding to Satiation
Endocrinology, December 1, 2006; 147(12): 5786 - 5797.
[Abstract] [Full Text] [PDF]

Home page
 J PsychopharmacolHome page
G. P. Reynolds, M. J. Hill, and S. L. Kirk
The 5-HT2C receptor and antipsychoticinduced weight gain - mechanisms and genetics
J Psychopharmacol, July 1, 2006; 20(4_suppl): 15 - 18.
[Abstract] [PDF]

Home page
 J. Neurosci.Home page
H. Cui, F. Cai, and D. D. Belsham
Anorexigenic Hormones Leptin, Insulin, and {alpha}-Melanocyte-Stimulating Hormone Directly Induce Neurotensin (NT) Gene Expression in Novel NT-Expressing Cell Models
J. Neurosci., October 12, 2005; 25(41): 9497 - 9506.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
H. Zheng, L. M. Patterson, C. B. Phifer, and H.-R. Berthoud
Brain stem melanocortinergic modulation of meal size and identification of hypothalamic POMC projections
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2005; 289(1): R247 - R258.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
V. C. Russo, S. Metaxas, K. Kobayashi, M. Harris, and G. A. Werther
Antiapoptotic Effects of Leptin in Human Neuroblastoma Cells
Endocrinology, September 1, 2004; 145(9): 4103 - 4112.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Belouzard, D. Delcroix, and Y. Rouille
Low Levels of Expression of Leptin Receptor at the Cell Surface Result from Constitutive Endocytosis and Intracellular Retention in the Biosynthetic Pathway
J. Biol. Chem., July 2, 2004; 279(27): 28499 - 28508.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
L. Huo, H. Munzberg, E. A. Nillni, and C. Bjorbaek
Role of Signal Transducer and Activator of Transcription 3 in Regulation of Hypothalamic trh Gene Expression by Leptin
Endocrinology, May 1, 2004; 145(5): 2516 - 2523.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
Y. Xing, A. Resch, and C. Lee
The Multiassembly Problem: Reconstructing Multiple Transcript Isoforms From EST Fragment Mixtures
Genome Res., March 1, 2004; 14(3): 426 - 441.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-N. Huan, J. Li, Y. Han, K. Chen, N. Wu, and A. Z. Zhao
Adipocyte-selective Reduction of the Leptin Receptors Induced by Antisense RNA Leads to Increased Adiposity, Dyslipidemia, and Insulin Resistance
J. Biol. Chem., November 14, 2003; 278(46): 45638 - 45650.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. Michel, B. E. Levin, and A. A. Dunn-Meynell
Stress facilitates body weight gain in genetically predisposed rats on medium-fat diet
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2003; 285(4): R791 - R799.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. Munzberg, L. Huo, E. A. Nillni, A. N. Hollenberg, and C. Bjorbaek
Role of Signal Transducer and Activator of Transcription 3 in Regulation of Hypothalamic Proopiomelanocortin Gene Expression by Leptin
Endocrinology, May 1, 2003; 144(5): 2121 - 2131.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. C. Love, J. Kochran, R. L. Cathey, S.-H. Shin, and J. A. Hanover
Mitochondrial and nucleocytoplasmic targeting of O-linked GlcNAc transferase
J. Cell Sci., February 15, 2003; 116(4): 647 - 654.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
K. D. Niswender, C. D. Morrison, D. J. Clegg, R. Olson, D. G. Baskin, M. G. Myers Jr., R. J. Seeley, and M. W. Schwartz
Insulin Activation of Phosphatidylinositol 3-Kinase in the Hypothalamic Arcuate Nucleus: A Key Mediator of Insulin-Induced Anorexia
Diabetes, February 1, 2003; 52(2): 227 - 231.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
R. D. Richardson, K. Omachi, R. Kermani, and S. C. Woods
Intraventricular insulin potentiates the anorexic effect of corticotropin releasing hormone in rats
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2002; 283(6): R1321 - R1326.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. Fekete, S. Sarkar, W. M. Rand, J. W. Harney, C. H. Emerson, A. C. Bianco, and R. M. Lechan
Agouti-Related Protein (AGRP) Has a Central Inhibitory Action on the Hypothalamic-Pituitary-Thyroid (HPT) Axis; Comparisons between the Effect of AGRP and Neuropeptide Y on Energy Homeostasis and the HPT Axis
Endocrinology, October 1, 2002; 143(10): 3846 - 3853.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
J Barrenetxe, A C Villaro, L Guembe, I Pascual, M Munoz-Navas, A Barber, and M P Lostao
Distribution of the long leptin receptor isoform in brush border, basolateral membrane, and cytoplasm of enterocytes
Gut, June 1, 2002; 50(6): 797 - 802.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. J. Grill, M. W. Schwartz, J. M. Kaplan, J. S. Foxhall, J. Breininger, and D. G. Baskin
Evidence That the Caudal Brainstem Is a Target for the Inhibitory Effect of Leptin on Food Intake
Endocrinology, January 1, 2002; 143(1): 239 - 246.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. Fekete, J. Kelly, E. Mihaly, S. Sarkar, W. M. Rand, G. Legradi, C. H. Emerson, and R. M. Lechan
Neuropeptide Y Has a Central Inhibitory Action on the Hypothalamic-Pituitary-Thyroid Axis
Endocrinology, June 1, 2001; 142(6): 2606 - 2613.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
T. Hubschle, E. Thom, A. Watson, J. Roth, S. Klaus, and W. Meyerhof
Leptin-Induced Nuclear Translocation of STAT3 Immunoreactivity in Hypothalamic Nuclei Involved in Body Weight Regulation
J. Neurosci., April 1, 2001; 21(7): 2413 - 2424.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. Shimizu-Albergine, D. L. Ippolito, and J. A. Beavo
Downregulation of Fasting-Induced cAMP Response Element-Mediated Gene Induction by Leptin in Neuropeptide Y Neurons of the Arcuate Nucleus
J. Neurosci., February 15, 2001; 21(4): 1238 - 1246.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
J. F. Breininger and D. G. Baskin
Fluorescence In Situ Hybridization of Scarce Leptin Receptor mRNA using the Enzyme-Labeled Fluorescent Substrate Method and Tyramide Signal Amplification
J. Histochem. Cytochem., December 1, 2000; 48(12): 1593 - 1600.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
J. E. McMinn, D. K. Sindelar, P. J. Havel, and M. W. Schwartz
Leptin Deficiency Induced by Fasting Impairs the Satiety Response to Cholecystokinin
Endocrinology, December 1, 2000; 141(12): 4442 - 4448.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M.-L. Hakansson-Ovesjo, M. Collin, and B. Meister
Down-Regulated STAT3 Messenger Ribonucleic Acid and STAT3 Protein in the Hypothalamic Arcuate Nucleus of the Obese Leptin-Deficient (ob/ob) Mouse
Endocrinology, November 1, 2000; 141(11): 3946 - 3955.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. E. McMinn, C. W. Wilkinson, P. J. Havel, S. C. Woods, and M. W. Schwartz
Effect of intracerebroventricular alpha -MSH on food intake, adiposity, c-Fos induction, and neuropeptide expression
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2000; 279(2): R695 - R703.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
J. Alfer, F. Muller-Schottle, I. Classen-Linke, U. von Rango, L. Happel, K. Beier-Hellwig, W. Rath, and H. M. Beier
The endometrium as a novel target for leptin: differences in fertility and subfertility
Mol. Hum. Reprod., July 1, 2000; 6(7): 595 - 601.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. A. Ammar, F. Sederholm, T. R. Saito, A. J. W. Scheurink, A. E. Johnson, and P. Sodersten
NPY-leptin: opposing effects on appetitive and consummatory ingestive behavior and sexual behavior
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2000; 278(6): R1627 - R1633.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
B. V. Zlokovic, S. Jovanovic, W. Miao, S. Samara, S. Verma, and C. L. Farrell
Differential Regulation of Leptin Transport by the Choroid Plexus and Blood-Brain Barrier and High Affinity Transport Systems for Entry into Hypothalamus and Across the Blood-Cerebrospinal Fluid Barrier
Endocrinology, April 1, 2000; 141(4): 1434 - 1441.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. Matsuda, I. Yokota, Y. Tsuruo, T. Murakami, K. Ishimura, K. Shima, and Y. Kuroda
Developmental Changes in Long-Form Leptin Receptor Expression and Localization in Rat Brain
Endocrinology, November 1, 1999; 140(11): 5233 - 5238.
[Abstract] [Full Text]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1999

 
Purchase HCS Short Course Manual on HCS site