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ARTICLE |
Three Distinct Classes of the 
-Subunit of the Nuclear Pore-targeting Complex (Importin-) Are Differentially Expressed in Adult Mouse Tissues
Yasuhiro Kameia,
Shunsuke Yubab,
Tatsuo Nakayamaa, and
Yoshihiro Yonedab
a Department of Biochemistry, Miyazaki Medical College, Miyazaki, Japan
b Department of Anatomy and Cell Biology, Osaka University Medical School, Suita, Osaka, Japan
Correspondence to: Yoshihiro Yoneda, Dept. of Anatomy and Cell Biology, Osaka Univ. Medical School, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan.
The process of active nuclear protein transport is mediated by the nuclear localization signal (NLS). An NLS-containing karyophile forms a stable complex, termed the nuclear pore-targeting complex, to target nuclear pores. The -subunit of the complex (importin-) binds to the NLS and the ß-subunit (importin-ß) carries the -subunit, bound to the NLS substrate, into the nucleus. To date, five mouse -subunits have been identified and classified into three subfamilies (-P, -Q, and -S). The expression of these -subunits and the ß-subunit in various adult mouse tissues was examined by immunoblotting and immunohistochemistry using antibodies specific for each subfamily of the -subunit or the ß-subunit. The ß-subunit was found to be ubiquitously expressed, whereas each subfamily of the -subunit showed a unique expression pattern in various tissues, especially in brain and testis. In brain, the expression of -P was not observed, whereas -S was significantly expressed in Purkinje cells, and pyramidal cells of the hippocampus and cerebral cortex. In testis, -P was expressed predominantly in primary spermatocytes, whereas -Q was found mainly in Leydig cells. Expression of -S was detected in almost all cells in convoluted seminiferous tubules and Leydig cells to a similar extent. These results suggest that nuclear protein import may be controlled in a tissue-specific manner by -subunit family proteins. (J Histochem Cytochem 47:363–372, 1999)
Key Words:
importin-, importin-ß, nuclear protein transport, nuclear localization signal, nuclear pore-targeting complex
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