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Journal of Histochemistry and Cytochemistry, Vol. 47, 393-400, March 1999, Copyright © 1999, The Histochemical Society, Inc.

ARTICLE

Lipopolysaccharide Treatment Modifies pH- and Cation-dependent Ecto-ATPase Activity of Endothelial Cells

Agnes Kittela
a Department of Cell Biology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary

Correspondence to: Agnes Kittel, Dept. of Cell Biology, Inst. of Experimental Medicine, Hungarian Academy of Sciences, PO Box 67, 1450 Budapest, Hungary.

The aim of this study was to investigate the distribution pattern of Ca2+- and Mg2+-dependent ecto-ATPases on the surface of rat brain capillary endothelial cells (ECs) in control and lipopolysaccharide (LPS)-treated animals. Ecto-ATPases in the membrane of vascular endothelial cells are suggested to play a crucial role in thromboregulation. Loss of this enzyme activity after oxidative stress and upregulation of the enzyme chain hydrolyzing extracellular ATP after transient forebrain ischemia have also been reported. We used histochemistry to localize the activities of this enzyme on ECs and found pH- and cation-dependent changes in the localization of enzyme activity both in control and in LPS-treated animals. These findings suggest the presence of more than one ecto-ATPase enzyme on the surface of rat capillary ECs. The different behavior of ECs after LPS treatment is the target of further investigations. The increased ecto-nucleotidase activity might play a role in nucleotide-mediated cellular responses after bacterial infections. (J Histochem Cytochem 47:393–399, 1999)

Key Words: ecto-ATPase, CD39, capillary endothelial cell, LPS treatment


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