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Journal of Histochemistry and Cytochemistry, Vol. 47, 481-488, April 1999, Copyright © 1999, The Histochemical Society, Inc.

ARTICLE

Keratan Sulfate Glycosaminoglycans in Murine Eosinophil-specific Granules

Jun Ohmoria, Yukifumi Nawab, Dan-Ha Yanga, Shinichiro Tsuyamaa, and Fusayoshi Murataa
a Department of Anatomy, Kagoshima University, Faculty of Medicine, Sakuragaoka, Kagoshima
b Department of Parasitology, Miyazaki Medical College, Kiyotake, Miyazaki

Correspondence to: Jun Ohmori, Dept. of Anatomy, Kagoshima University, Faculty of Medicine, 8-35-1 Sakuragaoka, Kagoshima 890-8520, Japan.

We examined the presence of sialyl glycoconjugates in specific granules from murine bone marrow eosinophils. Lectin cytochemistry using Maackia amurensis lectin II (MAL II) specific for sialyl {alpha}-2,3 galactose residues demonstrated positive labeling in both immature and mature specific granules. Pretreatment with Clostridium neuraminidase or keratanase II eliminated the positive labeling of MAL II in the specific granules. High iron diamine–thiocarbohydrazide–silver proteinate physical development (HID-TCH-SP-PD) staining, which is specific for sulfated glycoconjugates, also positively labeled immature specific granules lacking crystalloids but not mature granules with crystalloids. Pretreatment with a combination of chondroitinase ABC and keratanase, or a combination of chondroitinase ABC and keratanase II, eliminated the positive labeling obtained with HID-TCH-SP-PD. These results indicate that the sialyl residues detected by MAL II are expressed as terminal sugar residues of keratan sulfate proteoglycan, which appears to be of the corneal type in view of its sensitivity to keratanase and keratanase II. (J Histochem Cytochem 47:481–488, 1999)

Key Words: keratan sulfate, acidic glycoconjugates, eosinophil-specific granules, mouse


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