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Journal of Histochemistry and Cytochemistry, Vol. 47, 507-516, April 1999, Copyright © 1999, The Histochemical Society, Inc.

ARTICLE

Characterization of a Specific Antibody to the Rat Angiotensin II AT1 Receptor

Michelle E. Gilesa, Ross T. Fernleya, Yoshio Nakamuraa, Ingrid Moellera, G. Peter Aldreda, Tania Ferraroa, Jennifer D. Penschowa, Michael J. McKinleya, and Brian J. Oldfielda
a The Howard Florey Institute of Experimental Physiology and Medicine, Parkville, Victoria, Australia

Correspondence to: Brian J. Oldfield, Howard Florey Inst. of Experimental Physiology and Medicine, Univ. of Melbourne, Parkville, Victoria 3052, Australia.

We raised a polyclonal antibody against a decapeptide corresponding to the carboxyl terminus of the rat angiotensin II AT1 receptor. This antibody was demonstrated to be specific for the rat receptor according to a number of approaches. These included (a) the ultrastructural localization of immunogold-labeled receptor on the surfaces of zona glomerulosa cells in the adrenal cortex, (b) the specific labeling of Chinese hamster ovarian (CHO) cells transfected with AT1 receptors, (c) the identification of a specific band on Western blots, (d) the immunocytochemical co-localization of angiotensin receptors on neurons in the lamina terminalis of the brain shown to be responsive to circulating angiotensin II, as shown by the expression of c-fos, and (e) the correlation between the expression of the mRNA of the AT1 receptor and AT1 receptor immunoreactivity. (J Histochem Cytochem 47:507–515, 1999)

Key Words: angiotensin II, AT1 receptor, lamina terminalis


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