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Journal of Histochemistry and Cytochemistry, Vol. 47, 575-582, May 1999, Copyright © 1999, The Histochemical Society, Inc.

REVIEW

Oxygen Insensitivity of the Histochemical Assay of Glucose-6-Phosphate Dehydrogenase Activity for the Discrimination Between Nonmalignant and Malignant Cells

Bernard E.M. Van Driela and Cornelis J.F. Van Noordena
a Academic Medical Center, University of Amsterdam, Laboratory of Cell Biology and Histology, Amsterdam, The Netherlands

Correspondence to: Cornelis J.F. Van Noorden, Lab. of Cell Biology and Histology, Academic Medical Center, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.

We review here the oxygen insensitivity of the histochemical assay of glucose-6-phosphate dehydrogenase (G6PDH) activity to detect cancer cells. This inexpensive and rapid assay can be performed within half an hour. Discrimination between cancerous and noncancerous cells is based on a combination of elevated G6PDH activity, decreased superoxide dismutase (SOD) activity, and decreased lipid peroxidation in cancer cells. The test discriminates between adenomas and carcinomas of the colon with a certainty of >80% and has a high prognostic value for survival of colon cancer patients. Pancreatitis and pancreatic cancer are discriminated with a certainty of 100%. Therefore, the test can be applied by pathologists to provide additional information in difficult cases of diagnosis of cancer and for prognosis. (J Histochem Cytochem 47:575–582, 1999)

Key Words: cancer, malignancy, neoplasm, diagnosis, prognosis, glucose-6-phosphate dehydrogenase, enzyme histochemistry, quantitation


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