Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tran, D.
Right arrow Articles by Guillon, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tran, D.
Right arrow Articles by Guillon, G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 47, 601-616, May 1999, Copyright © 1999, The Histochemical Society, Inc.

ARTICLE

Distribution of Signaling Molecules Involved in Vasopressin-induced Ca2+ Mobilization in Rat Hepatocyte Multiplets

Dien Trana, Nicole Stellya, Thierry Tordjmanna, Thierry Durrouxb, Marie Noelle Dufourb, Arlette Forchionic, René Seyerb, Michel Clareta, and Gilles Guillonb
a INSERM U442, IFR-FR 46, Université Paris Sud, Orsay, France
b INSERM U469 Université Montpellier I, Montpellier, France
c Service d'Imagerie Cellulaire, Université Paris Sud, Orsay, France

Correspondence to: Michel Claret, Signalisation Cellulaire et Calcium, INSERM U442, IFR-FR 46, Université Paris Sud, bât. 443, 91405 Orsay, France.

In freshly isolated rat hepatocyte multiplets, Ca2+ signals in response to vasopressin are highly organized. In this study we used specific probes to visualize, by fluorescence and confocal microscopy, the main signaling molecules involved in vasopressin-mediated Ca2+ responses. V1a receptors were detected with a novel fluorescent antagonist, Rhm8-PVA. The G{alpha}q/G{alpha}11, PLCß3, PIP2, and InsP3 receptors were detected with specific antibodies. V1a vasopressin receptors and PIP2 were associated with the basolateral membrane and were not detected in the bile canalicular domain. G{alpha}q/G{alpha}11, PLCß3, and InsP3 receptors were associated with the basolateral membrane and also with other intracellular structures. We used double labeling, Western blotting, and drugs (cytochalasin D, colchicine) known to disorganize the cytoskeleton to demonstrate the partial co-localization of G{alpha}q/G{alpha}11 with F-actin. (J Histochem Cytochem 47:601–616, 1999)

Key Words: hepatocyte multiplets, fluorescent antagonist, V1a receptors, antibodies, G{alpha}q/G{alpha}11, cytoskeleton, fluorescence and confocal micoscopy, Western blotting


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
F. A. Wald, A. S. Oriolo, M. L. Casanova, and P. J.I. Salas
Intermediate Filaments Interact with Dormant Ezrin in Intestinal Epithelial Cells
Mol. Biol. Cell, September 1, 2005; 16(9): 4096 - 4107.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
J. C. SAEZ, V. M. BERTHOUD, M. C. BRANES, A. D. MARTINEZ, and E. C. BEYER
Plasma Membrane Channels Formed by Connexins: Their Regulation and Functions
Physiol Rev, October 1, 2003; 83(4): 1359 - 1400.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S. Miserey-Lenkei, Z. Lenkei, C. Parnot, P. Corvol, and E. Clauser
A Functional Enhanced Green Fluorescent Protein (EGFP)-Tagged Angiotensin II AT1A Receptor Recruits the Endogenous G{{alpha}}q/11 Protein to the Membrane and Induces Its Specific Internalization Independently of Receptor- G Protein Coupling in HEK-293 Cells
Mol. Endocrinol., February 1, 2001; 15(2): 294 - 307.
[Abstract] [Full Text]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1999