ARTICLE

Tissue and Cell Distribution of the Multidrug Resistance-Associated Protein (MRP) in Mouse Intestine and Kidney

Correspondence to: Alain Vandewalle, INSERM U478, Faculté de Médecine Xavier Bichat, BP 416, 75870 Paris Cedex 18, France. E-mail: vandewal@bichat.inserm.fr

The multidrug resistance-associated protein (MRP) that is involved in drug resistance and the export of glutathione-conjugated substrates may not have the same epithelial cell membrane distribution as the P-glycoprotein encoded by the MDR gene. Because intestinal and kidney epithelial cells are polarized cells endowed distinct secreting and absorptive ion and protein transport capacities, we investigated the tissue and cell distribution of MRP in adult mouse small intestine, colon, and kidney by immunohistochemistry. Western blot analyses revealed the 190-kD MRP protein in these tissues. MRP was found in the basolateral membranes of intestinal crypt cells, mainly Paneth cells, but not in differentiated enterocytes. All the cells lining the crypt–villous axis of the colon wall contained MRP. MRP was found in the glomeruli, ascending limb cells, and basolateral membranes of the distal and collecting tubule cells of the kidney but not in proximal tubule cells. Cultured mouse intestinal m-IC_cl2 cells and renal distal mpkDCT cells that have retained the features typical of intestinal crypt and renal distal epithelial cells, respectively, also possess MRP in their basolateral membranes. The patterns of subcellular and cellular distribution indicate that MRP may have a specific role in the basolateral transport of endogenous compounds in Paneth, renal distal, and collecting tubule cells. (J Histochem Cytochem 47:757–767, 1999)

Key Words: MRP, small intestine, Paneth cells, colon, kidney, immunocytochemistry

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. C. Bridges, L. Joshee, and R. K. Zalups MRP2 and the DMPS- and DMSA-Mediated Elimination of Mercury in TR- and Control Rats Exposed to Thiol S-Conjugates of Inorganic Mercury Toxicol. Sci., September 1, 2008; 105(1): 211 - 220. [Abstract] [Full Text] [PDF]

				C. C. Bridges, L. Joshee, and R. K. Zalups Multidrug Resistance Proteins and the Renal Elimination of Inorganic Mercury Mediated by 2,3-Dimercaptopropane-1-Sulfonic Acid and Meso-2,3-dimercaptosuccinic Acid J. Pharmacol. Exp. Ther., January 1, 2008; 324(1): 383 - 390. [Abstract] [Full Text] [PDF]

				R. G. Deeley, C. Westlake, and S. P. C. Cole Transmembrane Transport of Endo- and Xenobiotics by Mammalian ATP-Binding Cassette Multidrug Resistance Proteins. Physiol Rev, July 1, 2006; 86(3): 849 - 899. [Abstract] [Full Text] [PDF]

				S. Dallas, D. S. Miller, and R. Bendayan Multidrug resistance-associated proteins: expression and function in the central nervous system. Pharmacol. Rev., June 1, 2006; 58(2): 140 - 161. [Abstract] [Full Text] [PDF]

				H. M. Prime-Chapman, R. A. Fearn, A. E. Cooper, V. Moore, and B. H. Hirst Differential Multidrug Resistance-Associated Protein 1 through 6 Isoform Expression and Function in Human Intestinal Epithelial Caco-2 Cells J. Pharmacol. Exp. Ther., November 1, 2004; 311(2): 476 - 484. [Abstract] [Full Text] [PDF]

				J. Narukawa, H. Inoue, S. Kato, and H. Yokota GLUCURONIDATION OF 1-NAPHTHOL AND EXCRETION INTO THE VEIN IN PERFUSED RAT KIDNEY Drug Metab. Dispos., July 1, 2004; 32(7): 758 - 761. [Abstract] [Full Text] [PDF]

				J. C. de Jong, P. H. G. M. Willems, M. Goossens, A. Vandewalle, L. P. W. J. van den Heuvel, N. V. A. M. Knoers, and R. J. M. Bindels Effects of chemical chaperones on partially retarded NaCl cotransporter mutants associated with Gitelman's syndrome in a mouse cortical collecting duct cell line Nephrol. Dial. Transplant., May 1, 2004; 19(5): 1069 - 1076. [Abstract] [Full Text] [PDF]

				R. J. W. Diepens, E. den Dekker, M. Bens, A. F. Weidema, A. Vandewalle, R. J. M. Bindels, and J. G. J. Hoenderop Characterization of a murine renal distal convoluted tubule cell line for the study of transcellular calcium transport Am J Physiol Renal Physiol, March 1, 2004; 286(3): F483 - F489. [Abstract] [Full Text]

				D. E. Atkinson, S. L. Greenwood, C. P. Sibley, J. D. Glazier, and L. J. Fairbairn Role of MDR1 and MRP1 in trophoblast cells, elucidated using retroviral gene transfer Am J Physiol Cell Physiol, September 1, 2003; 285(3): C584 - C591. [Abstract] [Full Text] [PDF]

				T. Li, K. Ito, and T. Horie Transport of fluorescein methotrexate by multidrug resistance-associated protein 3 in IEC-6 cells Am J Physiol Gastrointest Liver Physiol, August 8, 2003; 285(3): G602 - G610. [Abstract] [Full Text] [PDF]

				K. Beck, K. Hayashi, B. Nishiguchi, O. Le Saux, M. Hayashi, and C. D. Boyd The Distribution of Abcc6 in Normal Mouse Tissues Suggests Multiple Functions for this ABC Transporter J. Histochem. Cytochem., July 1, 2003; 51(7): 887 - 902. [Abstract] [Full Text] [PDF]

				Y. Kato, K. Kuge, H. Kusuhara, P. J. Meier, and Y. Sugiyama Gender Difference in the Urinary Excretion of Organic Anions in Rats J. Pharmacol. Exp. Ther., August 1, 2002; 302(2): 483 - 489. [Abstract] [Full Text] [PDF]

				C. Veau, L. Faivre, S. Tardivel, M. Soursac, H. Banide, B. Lacour, and R. Farinotti Effect of Interleukin-2 on Intestinal P-glycoprotein Expression and Functionality in Mice J. Pharmacol. Exp. Ther., August 1, 2002; 302(2): 742 - 750. [Abstract] [Full Text] [PDF]

				P. A. Kothare and C. L. Zimmerman Intestinal Metabolism: The Role of Enzyme Localization in Phenol Metabolite Kinetics Drug Metab. Dispos., May 1, 2002; 30(5): 586 - 594. [Abstract] [Full Text] [PDF]

				D. Rost, S. Mahner, Y. Sugiyama, and W. Stremmel Expression and localization of the multidrug resistance-associated protein 3 in rat small and large intestine Am J Physiol Gastrointest Liver Physiol, April 1, 2002; 282(4): G720 - G726. [Abstract] [Full Text] [PDF]

				A. Rajgopal, E. E. Sierra, R. Zhao, and I. D. Goldman Expression of the reduced folate carrier SLC19A1 in IEC-6 cells results in two distinct transport activities Am J Physiol Cell Physiol, November 1, 2001; 281(5): C1579 - C1586. [Abstract] [Full Text] [PDF]

				J. Taipalensuu, H. Tornblom, G. Lindberg, C. Einarsson, F. Sjoqvist, H. Melhus, P. Garberg, B. Sjostrom, B. Lundgren, and P. Artursson Correlation of Gene Expression of Ten Drug Efflux Proteins of the ATP-Binding Cassette Transporter Family in Normal Human Jejunum and in Human Intestinal Epithelial Caco-2 Cell Monolayers J. Pharmacol. Exp. Ther., October 1, 2001; 299(1): 164 - 170. [Abstract] [Full Text] [PDF]

				D. Laouari, R. Yang, C. Veau, I. Blanke, and G. Friedlander Two apical multidrug transporters, P-gp and MRP2, are differently altered in chronic renal failure Am J Physiol Renal Physiol, April 1, 2001; 280(4): F636 - F645. [Abstract] [Full Text] [PDF]

				J.-P. Duong Van Huyen, M. Bens, J. Teulon, and A. Vandewalle Vasopressin-stimulated chloride transport in transimmortalized mouse cell lines derived from the distal convoluted tubule and cortical and inner medullary collecting ducts Nephrol. Dial. Transplant., February 1, 2001; 16(2): 238 - 245. [Abstract] [Full Text] [PDF]

				R. A. M. H. Van Aubel, R. Masereeuw, and F. G. M. Russel Molecular pharmacology of renal organic anion transporters Am J Physiol Renal Physiol, August 1, 2000; 279(2): F216 - F232. [Abstract] [Full Text] [PDF]

				A. D. Mottino, T. Hoffman, L. Jennes, and M. Vore Expression and Localization of Multidrug Resistant Protein mrp2 in Rat Small Intestine J. Pharmacol. Exp. Ther., June 1, 2000; 293(3): 717 - 723. [Abstract] [Full Text]

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1999