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Journal of Histochemistry and Cytochemistry, Vol. 47, 787-798, June 1999, Copyright © 1999, The Histochemical Society, Inc.

ARTICLE

Expression of Embryonic Fibronectin Isoform EIIIA Parallels {alpha}-Smooth Muscle Actin in Maturing and Diseased Kidney

Veronique L. Barnesa, John Musac, Ronda J. Mitchella, and Jeffrey L. Barnesa,b
a Department of Medicine, Division of Nephrology, University of Texas Health Science Center, San Antonio, Texas
b Medical Research Service, Audie Murphy Memorial Veterans Administration Hospital, San Antonio, Texas
c Department of Nephrology, Wilford Hall USAF Hospital, San Antonio, Texas

Correspondence to: Jeffrey L. Barnes, Dept. of Medicine, Div. of Nephrology, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78284.

In this study we examined if an association exists between expression of an alternatively spliced "embryonic" fibronectin isoform EIIIA (Fn-EIIIA) and {alpha}-smooth muscle actin ({alpha}-SMA) in the maturing and adult rat kidney and in two unrelated models of glomerular disease, passive accelerated anti-glomerular basement membrane (GBM) nephritis and Habu venom (HV)-induced proliferative glomerulonephritis, using immunohistochemistry and in situ hybridization. Fn-EIIIA and {alpha}-SMA proteins were abundantly expressed in mesangium and in periglomerular and peritubular interstitium of 20-day embryonic and 7-day (D-7) postnatal kidneys in regions of tubule and glomerular development. Staining was markedly reduced in these structures in maturing juvenile (D-14) kidney and was largely lost in adult kidney. Expression of Fn-EIIIA and {alpha}-SMA was reinitiated in the mesangium and the periglomerular and peritubular interstitium in both models and was also observed in glomerular crescents in anti-GBM nephritis. Increased expression of Fn-EIIIA mRNA by in situ hybridization corresponded to the localization of protein staining. Dual labeling experiments verified co-localization of Fn-EIIIA and {alpha}-SMA, showing a strong correlation of staining between location and staining intensity during kidney development, maturation, and disease. Expression of EIIIA mRNA corresponded to protein expression in developing and diseased kidneys and was lost in adult kidney. These studies show a recapitulation of the co-expression of Fn-EIIIA and {alpha}-SMA in anti-GBM disease and suggest a functional link for these two proteins. (J Histochem Cytochem 47:787–797, 1999)

Key Words: fibronectin, alternative splicing, smooth muscle actin, mesangium, glomerulonephritis, interstitial nephritis, fibrosis


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