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Journal of Histochemistry and Cytochemistry, Vol. 47, 833-836, June 1999, Copyright © 1999, The Histochemical Society, Inc.

BRIEF REPORT

Intranuclear Distribution of 8-hydroxy-2'-deoxyguanosine: An Immunocytochemical Study

Shinya Toyokunia, Yoko Iwasaa, Shohei Kondoa, Tomoyuki Tanakaa, Hirotomo Ochib, and Hiroshi Hiaia
a Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto, Japan
b Japan Institute for the Control of Aging, Fukuroi, Shizuoka, Japan

Correspondence to: Shinya Toyokuni, Dept. of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto Univ., Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.

We used immunofluorescence staining (monoclonal antibody N45.1) with cytological imprinting to study changes in the intranuclear distribution of 8-hydroxy-2'-deoxyguanosine in renal cells of male Wistar rats after oxidative stress by ferric nitrilotriacetate. In the control proximal tubule cells, small spherical signals were uniformly distributed throughout the nuclei. Under oxidative stress, immunofluorescence intensity was increased, especially near nuclear membrane. In cells with nuclear shrinkage or deformity, intense, diffuse signals throughout the nuclei were observed. Our results suggest that specific nuclear sites are vulnerable to oxidative DNA damage and that diffuse intense signals precede cell death after oxidative stress. (J Histochem Cytochem 47:833–835, 1999)

Key Words: 8-hydroxy-2'-deoxyguanosine, immunofluorescent staining, oxidative DNA damage, kidney, imprint cytology, ferric nitrilotriacetate


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