Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mentzel, S.
Right arrow Articles by Assmann, K.J.M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mentzel, S.
Right arrow Articles by Assmann, K.J.M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 47, 871-880, July 1999, Copyright © 1999, The Histochemical Society, Inc.

ARTICLE

In Vivo Antibody-mediated Modulation of Aminopeptidase A in Mouse Proximal Tubular Epithelial Cells

S. Mentzela, H.B.P.M. Dijkmana, J.P.H.F. van Sona, J.F.M. Wetzelsb, and K.J.M. Assmanna
a Departments of Pathology, Division of Nephrology, University Hospital Nijmegen, Nijmegen, The Netherlands
b Internal Medicine, Division of Nephrology, University Hospital Nijmegen, Nijmegen, The Netherlands

Correspondence to: S. Mentzel, Dept. of Pathology, University Hospital Nijmegen, PO Box 9101, 6500 HB Nijmegen, The Netherlands.

Aminopeptidase A (APA) is one of the many renal hydrolases. In mouse kidney, APA is predominantly expressed on the brush borders and sparsely on the basolateral membranes of proximal tubular epithelial cells. However, when large amounts of monoclonal antibodies (MAbs) against APA were injected into mice, we observed strong binding of the MAbs to the basolateral membranes, whereas the MAbs bound only transiently to the brush borders of the proximal tubular epithelial cells. In parallel, APA itself disappeared from the brush borders by both endocytosis and shedding, whereas it was increasingly expressed on the basolateral sides. Using ultrastructural immunohistology, we found no evidence for transcellular transport of endocytosed APA to the basolateral side of the proximal tubular epithelial cells. The absence of transcellular transport was confirmed by experiments in which we used a low dose of the MAbs. Such a low dose did not result in binding of the MAbs to the brush borders and had no effect on the presence of APA in the brush borders of the proximal tubular epithelial cells. In these experiments we still could observe binding of the MAbs to the basolateral membranes in parallel with the local appearance of APA. In addition, treatment of mice with chlorpromazine, a calmodulin antagonist that interferes with cytoskeletal function, largely inhibited the MAb-induced modulation of APA. Our studies suggest that injection of MAbs to APA specifically interrupts the normal intracellular traffic of this enzyme in proximal tubular epithelial cells. This intracellular transport is dependent on the action of cytoskeletal proteins. (J Histochem Cytochem 47:871–880, 1999)

Key Words: mouse, kidney, antibody, aminopeptidase A, proximal tubular epithelial cells, redistribution, cytoskeleton, angiotensin II, chlorpromazine


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Am. Soc. Nephrol.Home page
M. E. Gerlofs-Nijland, K. J. M. Assmann, H. B. P. M. Dijkman, J. W.C. Dieker, J. P. H. F. van Son, S. Mentzel, J. P. van Kats, A. H. J. Danser, O. Smithies, P. J. T. A. Groenen, et al.
Albuminuria in Mice after Injection of Antibodies against Aminopeptidase A: Role of Angiotensin II
J. Am. Soc. Nephrol., December 1, 2001; 12(12): 2711 - 2720.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1999