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Journal of Histochemistry and Cytochemistry, Vol. 47, 969-972, July 1999, Copyright © 1999, The Histochemical Society, Inc.

TECHNICAL NOTE

Detection of Pathological Zinc Accumulation In Neurons: Methods for Autopsy, Biopsy, and Cultured Tissue

Sang Won Suha,c,f, Kathy Listiacka, Brent Bella, Jefferson Chena, Massoud Motamedia, David Silvab, Gorm Danscherc, William Whetselld, Richard Thompsone, and Christopher Fredericksona,f
a Center For Biomedical Engineering, University of Texas Medical Branch, Galveston, Texas
b Laboratory for Zinc Neurobiology, MicroFab Technologies, Inc., Plano, Texas
c Institute of Neurobiology, University of Aarhus, Aarhus, Denmark
d Department of Pathology, Vanderbilt School of Medicine, Nashville, Tennessee
e Department of Chemistry, University of Maryland at Baltimore, Baltimore, Maryland
f NeuroBioTex, Inc., Galveston, Texas

Correspondence to: Christopher Frederickson, NeuroBioTex, Inc., 921 Sealy & Smith Bldg., 200 University Boulevard, Galveston, TX 77550

It has been repeatedly shown that synaptically released zinc contributes to excitotoxic neuronal injury in ischemia, epilepsy, and mechanical head trauma. Such zinc-induced injury leaves an unmistakable "footprint" in the injured neurons, allowing an easy and unambiguous postmortem diagnosis. This footprint is the presence of weakly bound, histochemically reactive zinc in the cytoplasm of the perikaryon and proximal dendrites. Such staining appears to be a necessary and sufficient marker for zinc-induced neuronal injury. Here we show how to prepare and stain tissue from biopsy, autopsy, or experimental animal sources for maximal contrast and visibility of zinc-injured neurons. (J Histochem Cytochem 47:969–972, 1999)

Key Words: zinc, neuronal injury, perikarya, dendrites, staining


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