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Journal of Histochemistry and Cytochemistry, Vol. 47, 1149-1158, September 1999, Copyright © 1999, The Histochemical Society, Inc.

ARTICLE

Localization of MCC (Mutated in Colorectal Cancer) in Various Tissues of Mice and Its Involvement in Cell Differentiation

Takao Sendaa, Akihiko Matsumineb, Hiroyuki Yanaib, and Tetsu Akiyamab,c
a Department of Anatomy I, Nagoya University School of Medicine, Nagoya, Japan
b Department of Oncogene Research, Research Institute for Microbial Diseases, Osaka University, Suita, Japan
c Laboratory of Molecular and Genetic Information, Institute of Molecular and Biological Sciences, The University of Tokyo, Tokyo, Japan

Correspondence to: Takao Senda, Dept. of Anatomy I, Nagoya U. School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan.

Localization of the MCC (mutated in colorectal cancer) gene product, a cell cycle-regulating protein mutated in several colorectal tumors, in various mouse tissues was examined by immunohistochemistry and immunoelectron microscopy. MCC was localized on microvilli and in the apical cytoplasm in renal proximal tubule epithelial cells and pancreatic acinar cells. In hepatocytes, MCC was exclusively detected on microvilli. MCC was highly expressed in the cerebral cortex and the molecular layer of the cerebellar cortex and was partially associated with membrane organelles in neuronal elements. Adrenal chromaffin cells showed little expression of MCC. MCC was localized to the cell margins of ependymal cells, thyroid follicular cells, and anterior pituitary cells. In parotid acinar cells, only the apical surface was immunopositive. MCC was not expressed in skeletal and cardiac muscle. MCC was present at lateral cell borders in the duodenum and colon epithelium. In addition, the apical cytoplasm of colon epithelial cells exhibited intense immunoreactivity. The amount of MCC increased during differentiation of NGF-treated PC12 cells. In conclusion, MCC was expressed in differentiated cells and was associated with the plasma membrane and membrane organelles. In addition to the negative regulation of the cell cycle, MCC may be involved in cell differentiation. (J Histochem Cytochem 47:1149–1157, 1999)

Key Words: MCC, signaling pathway, cell cycle, cell differentiation, immunohistochemistry, immunoelectron microscopy


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