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Journal of Histochemistry and Cytochemistry, Vol. 48, 35-44, January 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

Behavior of Smooth Muscle Cells during Arterial Ductal Closure at Birth

Shin-ichiro Imamuraa, Toshio Nishikawac, Eriko Hiratsukaa, Atsuyoshi Takaob, and Rumiko Matsuokab
a Research Division, Tokyo Women's Medical University, Tokyo, Japan
b Department of Pediatric Cardiology, Tokyo Women's Medical University, Tokyo, Japan
c The Heart Institute of Japan, and Department of Surgical Pathology, Tokyo Women's Medical University, Tokyo, Japan

Correspondence to: Rumiko Matsuoka, Dept. of Pediatric Cardiology, The Heart Institute of Japan, Tokyo Women's Medical U., 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.

To determine which part of the smooth muscle cells (SMCs) of the ductus arteriosus (DA) contribute to duct closure after birth, we looked for areas in which SM2 myosin heavy chain (MHC) mRNA expression, which is associated with contraction of smooth muscle, and apoptosis could be detected in the DA during development. In situ hybridization revealed that the SM2 MHC mRNA was strongly positive in the longitudinally oriented SMCs and inner layer of the circularly oriented SMCs just before birth. Apoptotic cells were detected in the SMCs of the DA from 1 day after birth. Histochemical analysis using terminal deoxynucleotidyl transferase-mediated dUTP–digoxigenin nick end-labeling (TUNEL) revealed significant numbers of TUNEL-positive nuclei in the longitudinally oriented SMCs and the inner layer of the circularly oriented SMCs. Masson-stained sections showed that the TUNEL-positive area in the DA was replaced by connective tissue from 1 day after birth. These results suggest that the increase in the SM2 MHC mRNA expression and the induction of apoptosis are present at the same site in the media of the DA. Therefore, the SMCs in this area may play an important role in duct constriction and remodeling of the vessel wall after birth. (J Histochem Cytochem 48:35–44, 2000)

Key Words: ductus arteriosus, duct closure, smooth muscle, myosin heavy chain isoform, apoptotic cell death, rabbit


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