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Journal of Histochemistry and Cytochemistry, Vol. 48, 89-94, January 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

Brush Border Myosin I (BBMI): A Basally Localized Transcript in Human Jejunal Enterocytes

Wei Lia, Jiyi Wangb, Lynne M. Coluccioc, Paul Matsudairad, and Richard J. Granda
a Divisions of Pediatric Gastroenterology/Nutrition, The Floating Hospital for Children, New England Medical Center Hospitals, Center for Gastroenterology Research on Absorptive and Secretory Processes, Tufts University School of Medicine
b Genetics, The Floating Hospital for Children, New England Medical Center Hospitals, Center for Gastroenterology Research on Absorptive and Secretory Processes, Tufts University School of Medicine
c Boston Biomedical Research Institute
d Whitehead Institute, Massachusetts Institute of Technology, Boston, Massachusetts

Correspondence to: Richard J. Grand, Dept. of Pediatrics, Box 213, New England Medical Center, 750 Washington Street, Boston, MA 02111.

To extend our recent observation that villin mRNA, encoding an apical microvillous protein, is dichotomously localized in the basal region of human enterocytes, we examined the localization of mRNAs for brush border myosin I (BBMI) and intestinal fimbrin (I-fim). In situ hybridization indicated that BBMI mRNA localized to the basal region of human enterocytes, whereas the mRNA for I-fim distributed diffusely. To facilitate study of potential mechanisms of mRNA targeting, we cloned a full-length cDNA for BBMI including its 5'- and 3'-untranslated regions (UTRs). This cDNA shares 86% sequence identity with bovine BBMI and 85% with rat BBMI. Sequence analysis revealed no obvious similarity between the 3'-UTRs of BBMI and villin. This study provides evidence of novel sorting pathways for intestinal microvillous cytoskeletal proteins. (J Histochem Cytochem 48:89–94, 2000)

Key Words: brush border myosin I, BBMI, intestinal fimbrin, I-fimbrin (I-plastin), mRNA sorting, enterocyte


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