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Journal of Histochemistry and Cytochemistry, Vol. 48, 1411-1420, October 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

Expression of Arachidonate 12-Lipoxygenase in Rat Tissues: A Possible Role in Glucagon Secretion

Hiroo Kawajiria, Daming Zhuanga, Na Qiaoa, Tanihiro Yoshimotoa, Miyuki Yamamotob, Shoichi Isekib, and Kazuyuki Hamaguchic
a Departments of Pharmacology, Kanazawa University School of Medicine, Kanazawa, Japan
b Anatomy I, Kanazawa University School of Medicine, Kanazawa, Japan
c Department of Internal Medicine, Oita Medical University, Hasama-machi, Oita, Japan

Correspondence to: Tanihiro Yoshimoto, Dept of Pharmacology, Kanazawa U. School of Medicine, 13-1 Takara-machi, Kanazawa, Ishikawa 920-8640, Japan. E-mail: yoshimot@med.kanazawa-u.ac.jp

There are three isoforms of arachidonate 12-lipoxygenase in mammals: platelet, leukocyte, and epidermal types. We found in this study that the leukocyte-type enzyme was present in rat pineal gland, lung, spleen, aorta, adrenal gland, spinal cord, and pancreas, as assessed by RT-PCR. Immunohistochemical analysis showed that the enzyme was localized in macrophages in lung and spleen, {alpha}-cells of pancreatic islet, zona glomerulosa cells of adrenal cortex, and neuronal cells of spinal cord and superior cervical ganglion. The presence of the 12-lipoxygenase in pancreatic {alpha}-cells was confirmed by glucagon staining in a consecutive section. We overexpressed the leukocyte-type 12-lipoxygenase cDNA in a glucagon-secreting {alpha}TC clone 6 cell line that had been established from a transgenic mouse. Glucagon secretion was stimulated by approximately twofold in the 12-lipoxygenase-expressing cells compared to the mock-transfected and original cells. The results suggest that the 12-lipoxygenase of the leukocyte type augments glucagon secretion from pancreatic islets. (J Histochem Cytochem 48:1411–1419, 2000)

Key Words: arachidonic acid, 12-lipoxygenase, immunohistochemistry, overexpression, pancreatic {alpha}-cell, glucagon


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