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Journal of Histochemistry and Cytochemistry, Vol. 48, 1469-1478, November 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

Distribution of Group II Phospholipase A2 Protein and mRNA in Rat Tissues

Kai M. Nymana, Pekka Ojalaa, Veli J.O. Lainea, and Timo J. Nevalainena
a Department of Pathology, University of Turku, Turku, Finland

Correspondence to: Timo J. Nevalainen, Dept. of Pathology, University of Turku, Kiinamyllynkatu 10, FIN-20520 Turku, Finland. E-mail: timo.nevalainen@utu.fi

Group II phospholipase A2 (PLA2) is an acute-phase protein and an important component of the host defense against bacteria. In this study we investigated the distribution of PLA2 protein by immunohistochemistry and the distribution of mRNA of PLA2 by Northern blotting and in situ hybridization in rat tissues. PLA2 protein was localized in the Paneth cells of the intestinal mucosa, chondrocytes and the matrix of cartilage, and megakaryocytes in the spleen. By Northern blotting, mRNA of PLA2 was found in the gastrointestinal tract, lung, heart, and spleen. By in situ hybridization, PLA2 mRNA was localized in the Paneth cells of the small intestinal mucosa but in no other cell types. Our results show specific distribution of PLA2 in a limited number of cell types in rat tissues. The reagents developed in this study (the anti-rat PLA2 antibody and probes for Northern blotting and in situ hybridization of mRNA of rat PLA2) will provide useful tools for future studies concerning the role of PLA2 in various experimental disease models. (J Histochem Cytochem 48:1469–1477, 2000)

Key Words: immunohistochemisty, in situ hybridization, Northern blotting, phospholipase A2, polyclonal antibody, rat, recombinant protein


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