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Journal of Histochemistry and Cytochemistry, Vol. 48, 1511-1520, November 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

Localization of Transcription Factor AP-1 Family Proteins in Ameloblast Nuclei of the Rat Incisor

Sumio Nishikawaa
a Department of Biology, Tsurumi University School of Dental Medicine, Yokohama, Japan

Correspondence to: Sumio Nishikawa, Dept. of Biology, Tsurumi U. School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan. E-mail: k01711@simail.ne.jp

We examined by immunocytochemistry the localization of the AP-1 family proteins c-Jun, JunB, JunD, c-Fos, FosB, Fra-1, and Fra-2 in rat incisor ameloblasts. Most of the antibodies against AP-1 family proteins, except for c-Fos-specific antibody, labeled ameloblast nuclei. The labeling intensity of the c-Jun, JunD, and Fra-2 antibodies was stronger than that of JunB, FosB, and Fra-1. Antibody reactivities of c-Jun, JunD, and Fra-2 were greatly enhanced during or after the transition zone. Furthermore, c-Jun antibodies labeled maturation ameloblasts in a cyclic pattern, which was correlated with ameloblast modulation. Disruption of ameloblast modulation by colchicine injection resulted in greatly decreased reactivity of the c-Jun antibody in the ameloblast nuclei of the maturation zone. Phospho-specific antibodies to c-Jun labeled ameloblast nuclei only weakly throughout the secretion, transition, and maturation zones. These results suggest that the stage-specific localization of AP-1 in ameloblasts is closely related to tooth enamel formation.

(J Histochem Cytochem 48:1511–1520, 2000)

Key Words: AP-1 proteins, ameloblast, enamel formation, rat incisor


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