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Journal of Histochemistry and Cytochemistry, Vol. 48, 1609-1616, December 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

A New Monoclonal Anti-CD3{varepsilon} Antibody Reactive on Paraffin Sections

Laeticia Alibauda, Régine Lloberaa, Talal Al Saatia, Michel Marcha, Georges Delsola, and Bent Rubina
a Unité de Physiopathologie Cellulaire et Moléculaire, CNRS-UPR 2163, Institut Claude de Preval, IFR 30, Chu de Purpan, Toulouse, France

Correspondence to: Georges Delsol, Unité de Physiopathologie Cellulaire et Moléculaire, CNRS-UPR 2163, Institut Claude de Preval, IFR 30, Chu de Purpan, 31059 Toulouse, Cedex 03, France. E-mail: rubin@immgen.cnrs.fr

We generated a new monoclonal antibody (MAb), F7.2.38, by immunizing mice with CD3{varepsilon}{gamma}{delta}/CD3{omega} complexes purified from human T-cells by OKT3 MAb–Sepharose affinity chromatography. Immunoprecipitation experiments and Western blotting analysis showed that MAb F7.2.38 recognized the CD3{varepsilon} chain in CD3{varepsilon} cDNA-transfected FOX B-cells and in various T-cell lines. Using flow cytometry on permeabilized or intact cells, the epitope was found to be located in the cytoplasmic tail of the CD3{varepsilon} chain. Immunohistochemical staining on paraffin-embedded sections showed that the reactivity of MAb F7.2.38 was comparable to that of the commercially available anti-CD3{varepsilon} polyclonal antibody. Of the 52 well-characterized T-cell lymphomas, 41 were positive for F7.2.38 (79%), whereas all 37 B-cell lymphomas and 69 non-lymphoid tumors were unreactive. This new anti-CD3{varepsilon} antibody would be particularly useful for phenotyping T-cell lymphomas on routinely processed paraffin-embedded tissue sections. (J Histochem Cytochem 48:1609–1616, 2000)

Key Words: monoclonal antibody, CD3{varepsilon} chain, T-cell lymphomas, T-cell receptor assembly, immunohistochemistry, paraffin sections


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