ARTICLE

Developmental Mucin Gene Expression in the Gastroduodenal Tract and Accessory Digestive Glands. II. Duodenum and Liver, Gallbladder, and Pancreas

Correspondence to: Nicole Porchet, Unité 377 INSERM, Place de Verdun, 59045 Lille Cedex, France. E-mail: jpa@lille.inserm.fr

Studies were undertaken to provide information regarding cell-specific expression of mucin genes and their relation to developmental and neoplastic patterns of epithelial cytodifferentiation. In situ hybridization was used to study mRNA expression of mucin genes in duodenum and accessory digestive glands (liver, gallbladder, pancreas) of 13 human embryos and fetuses (6.5–27 weeks' gestation), comparing these with normal and neoplastic adult tissues. These investigations demonstrated that the pattern of mucin gene expression in fetal duodenum reiterated the patterns we observed during gastric and intestinal ontogenesis, with MUC2 and MUC3 expression in the surface epithelium and MUC6 expression associated with the development of Brünner's glands. In embryonic liver, MUC3 was already expressed at 6.5 weeks of gestation in hepatoblasts. As in adults, MUC1, MUC2, MUC3, MUC5AC, MUC5B, and MUC6 were expressed in fetal gallbladder, whereas MUC4 was not. In contrast, MUC4 was strongly expressed in gallbladder adenocarcinomas. MUC5B and MUC6 were expressed in fetal pancreas, from 12 weeks and 26 weeks of gestation, respectively. Surprisingly, MUC3 which is strongly expressed in adult pancreas, was not detected in developmental pancreas. Taken together, these data show complex spatio–temporal regulation of the mucin genes and suggest a possible regulatory role for mucin gene products in gastroduodenal epithelial cell differentiation. (J Histochem Cytochem 48:1667–1676, 2000)

Key Words: mucin genes, development, differentiation, duodenum, biliary, pancreas, in situ hybridization

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				P. Chaturvedi, A. P. Singh, and S. K. Batra Structure, evolution, and biology of the MUC4 mucin FASEB J, April 1, 2008; 22(4): 966 - 981. [Abstract] [Full Text] [PDF]

				D. V. Gold, Z. Karanjawala, D. E. Modrak, D. M. Goldenberg, and R. H. Hruban PAM4-Reactive MUC1 Is a Biomarker for Early Pancreatic Adenocarcinoma Clin. Cancer Res., December 15, 2007; 13(24): 7380 - 7387. [Abstract] [Full Text] [PDF]

				N. Jonckheere, A. Vincent, M. Perrais, M.-P. Ducourouble, A. K.-v. Male, J.-P. Aubert, P. Pigny, K. L. Carraway, J.-N. Freund, I. B. Renes, et al. The Human Mucin MUC4 Is Transcriptionally Regulated by Caudal-related Homeobox, Hepatocyte Nuclear Factors, Forkhead Box A, and GATA Endodermal Transcription Factors in Epithelial Cancer Cells J. Biol. Chem., August 3, 2007; 282(31): 22638 - 22650. [Abstract] [Full Text] [PDF]

				P. Chaturvedi, A. P. Singh, N. Moniaux, S. Senapati, S. Chakraborty, J. L. Meza, and S. K. Batra MUC4 Mucin Potentiates Pancreatic Tumor Cell Proliferation, Survival, and Invasive Properties and Interferes with Its Interaction to Extracellular Matrix Proteins Mol. Cancer Res., April 1, 2007; 5(4): 309 - 320. [Abstract] [Full Text] [PDF]

				S. Blandamura, A. Parenti, B. Famengo, A. Canesso, P. Moschino, C. Pasquali, S. Pizzi, V. Guzzardo, and V. Ninfo Three cases of pancreatic serous cystadenoma and endocrine tumour J. Clin. Pathol., March 1, 2007; 60(3): 278 - 282. [Abstract] [Full Text] [PDF]

				H. H. Wang, N. H. Afdhal, S. J. Gendler, and D. Q.-H. Wang Lack of the intestinal Muc1 mucin impairs cholesterol uptake and absorption but not fatty acid uptake in Muc1-/- mice Am J Physiol Gastrointest Liver Physiol, September 1, 2004; 287(3): G547 - G554. [Abstract] [Full Text] [PDF]

				E Levi, D S Klimstra, N V Adsay, A Andea, and O Basturk MUC1 and MUC2 in pancreatic neoplasia J. Clin. Pathol., May 1, 2004; 57(5): 456 - 462. [Abstract] [Full Text] [PDF]

				H. H. Wang, N. H. Afdhal, S. J. Gendler, and D. Q-H. Wang Targeted disruption of the murine mucin gene 1 decreases susceptibility to cholesterol gallstone formation J. Lipid Res., March 1, 2004; 45(3): 438 - 447. [Abstract] [Full Text] [PDF]

				K. Nakajima, H. Ota, M. X. Zhang, K. Sano, T. Honda, K. Ishii, and J. Nakayama Expression of Gastric Gland Mucous Cell-Type Mucin in Normal and Neoplastic Human Tissues J. Histochem. Cytochem., December 1, 2003; 51(12): 1689 - 1698. [Abstract] [Full Text] [PDF]

				R. Drut and M. Drut Pancreatic Cystic Dysplasia (Dysgenesis) Presenting as a Surgical Pathology Specimen in a Patient with Multiple Malformations and Familial Ear Pits International Journal of Surgical Pathology, October 1, 2002; 10(4): 303 - 308. [Abstract] [PDF]

				M. Andrianifahanana, N. Moniaux, B. M. Schmied, J. Ringel, H. Friess, M. A. Hollingsworth, M. W. Buchler, J.-P. Aubert, and S. K. Batra Mucin (MUC) Gene Expression in Human Pancreatic Adenocarcinoma and Chronic Pancreatitis: A Potential Role of MUC4 as a Tumor Marker of Diagnostic Significance Clin. Cancer Res., December 1, 2001; 7(12): 4033 - 4040. [Abstract] [Full Text] [PDF]

				M-P Buisine, P Desreumaux, E Leteurtre, M-C Copin, J-F Colombel, N Porchet, and J-P Aubert Mucin gene expression in intestinal epithelial cells in Crohn's disease Gut, October 1, 2001; 49(4): 544 - 551. [Abstract] [Full Text] [PDF]

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2000