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PERSPECTIVES |
Specificity Controls for Immunocytochemical Methods
Richard W. Burryaa Department of Neuroscience, College of Medicine and Public Health, The Ohio State University, Columbus, Ohio
Correspondence to: Richard W. Burry, Dept of Neuroscience, Coll. of Medicine and Public Health, O.S.U., 333 W. Tenth Ave., Columbus, OH 43210.
Immunocytochemistry is used for antibody localization of proteins in cells and tissues. The specificity of the results depends on two independent criteria: the specificity of the antibody and of the method used. The antibody specificity is best determined by immunoblot and or immunoprecipitation. Absorption of the antibody with a protein does not determine that the antibody would have bound to the same protein in the tissue, and therefore is not a good control for antibody specificity. The specificity of the method is best determined by both a negative control, replacing the primary antibody with serum, and a positive control, using the antibody with cells known to contain the protein. With the increasing use of immunocytochemistry, it is important to be aware of the appropriate controls needed to show specificity of the labeling. (J Histochem Cytochem 48:163–165, 2000)
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