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Journal of Histochemistry and Cytochemistry, Vol. 48, 191-200, February 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

Distribution of I{kappa}B Proteins in Gastric Mucosa and Other Organs of Mouse and Gerbil

Masamichi Koyamaa, Samuel S. Spicera, and Bradley A. Schultea
a Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, South Carolina

Correspondence to: Bradley A. Schulte, Dept. of Pathology and Laboratory Medicine, Medical U. of South Carolina, 165 Ashley Avenue, Suite 309, PO Box 250908, Charleston, SC 29425. E-mail: schulteb@musc.edu

The NF-{kappa}B/I{kappa}B complex is a major transcription regulator of inflammatory and immune responses. Helicobacter pylori infection causes chronic inflammation in gastric mucosa by inducing dissociation of the inhibitory I{kappa}B protein from the complex with a resulting increased expression of interleukin (IL)-8. To clarify which of several known I{kappa}B proteins could be involved in this inflammatory response, we undertook immunohistochemical examination of normal mouse stomach as well as other murine tissues for comparison, using polyclonal antibodies specific for {alpha}-, ß-, {gamma}-, and {isin}-isoforms of I{kappa}B. The results showed strong immunoreactivity for the {alpha}-isoform in parietal cells and for the ß-isoform in pit cells of the stomach, along with the presence of these proteins in various other sites. Comparative staining revealed a similar but not identical distribution of I{kappa}B proteins in the Mongolian gerbil, a rodent model for H. pylori infection. The findings suggest that the {alpha}- and ß-isoforms are dominant I{kappa}B proteins in gastric parietal and foveolar cells, respectively, and point to a role for these transcription regulators in modulating pathological responses in stomach and other organs. (J Histochem Cytochem 48:191–199, 2000)

Key Words: transcription factors, I{kappa}B, immunohistochemistry, gastric pit cells, gastric parietal cells


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