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Journal of Histochemistry and Cytochemistry, Vol. 48, 201-210, February 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

Laminin Chains in Developing and Adult Human Myotendinous Junctions

Fatima Pedrosa–Domellöfa, Carl-Fredrik Tigerc, Ismo Virtanenb, Lars-Eric Thornella, and Donald Gullbergc
a Department of Integrative Medical Biology, Section for Anatomy, Umeå University, and Department of Musculoskeletal Research, National Institute for Working Life, Umeå, Sweden
b Department of Anatomy, Institute of Biomedicine, University of Helsinki, Helsinki, Finland
c Department of Cell & Molecular Biology, Uppsala University, Uppsala, Sweden

Correspondence to: Donald Gullberg, Dept. of Cell & Molecular Biology, Uppsala University, BMC Box 596, S-751 24 Uppsala, Sweden. E-mail: Donald.Gullberg@zoofys.uu.se (preferred for correspondence).

In addition to being the specialized site for transmission of force from the muscle to the tendon, the myotendinous junction (MTJ) also plays an important role in muscle splitting during morphogenesis. An early event in the formation of the MTJ is a regional deposition of basement membranes. We used immunocytochemistry to investigate the distribution of laminin chains during the development of MTJs in human limb muscle at 8–22 weeks of gestation (wg) and in adult MTJs. We used polyclonal antibodies and a new monoclonal antibody (MAb) against the human laminin {alpha}1 G4/G5 domains. At 8–10 wg, laminin {alpha}1 and laminin {alpha}5 chains were specifically localized to the MTJ. Laminin {alpha}1 chain remained restricted to the MTJ at 22 wg as the laminin ß2 chain had appeared, whereas the laminin {alpha}5 chain became deposited along the entire length of the myotubes from 12 wg. In the adult MTJ, only vestigial amounts of laminin {alpha}1 and laminin {alpha}5 chains could be detected. On the basis of co-distribution data, we speculate that laminin {alpha}1 chain in the forming MTJ undergoes an isoform switch from laminin 1 to laminin 3. Our data indicate a potentially important role for laminin {alpha}1 chain in skeletal muscle formation. (J Histochem Cytochem 48:201–209, 2000)

Key Words: myotendinous junction, laminin {alpha}1 chain, development, human, muscle


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