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Journal of Histochemistry and Cytochemistry, Vol. 48, 211-218, February 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

Localization of S-adenosylhomocysteine Hydrolase in the Rat Kidney

Doris Kloora, Wolfgang Stumvolla, Heide Schmidb, Jost Kömpfc, Andreas Mackd, and Hartmut Osswalda
a Department of Pharmacology, Faculty of Medicine, University of Tübingen,
b Department of Pathology, University of Tübingen
c Institute of Anthropology and Human Genetics, University of Tübingen
d Institute of Anatomy, University of Tübingen, Tübingen, Germany

Correspondence to: Doris Kloor, Dept. of Pharmacology, Wilhelmstr. 56, D-72074 Tübingen, Germany.

S-adenosylhomocysteine (SAH) hydrolase is a cytosolic enzyme present in the kidney. Enzyme activities of SAH hydrolase were measured in the kidney in isolated glomeruli and tubules. SAH hydrolase activity was 0.62 ± 0.02 mU/mg in the kidney, 0.32 ± 0.03 mU/mg in the glomeruli, and 0.50 ± 0.02 mU/mg in isolated tubules. Using immunohistochemical methods, we describe the localization of the enzyme SAH hydrolase in rat kidney with a highly specific antibody raised in rabbits against purified SAH hydrolase from bovine kidney. This antibody crossreacts to almost the same extent with the SAH hydrolase from different species such as rat, pig, and human. Using light microscopy, SAH hydrolase was visualized by the biotin–streptavidin–alkaline phosphatase immunohistochemical procedure. SAH hydrolase immunostaining was observed in glomeruli and in the epithelium of the proximal and distal tubules. The collecting ducts of the cortex and medulla were homogeneously stained. By using double immunofluorescence staining and two-channel immunofluorescence confocal laser scanning microscopy, we differentiated the glomerular cells (endothelium, mesangium, podocytes) and found intensive staining of podocytes. Our results show that the enzyme SAH hydrolase is found ubiquitously in the rat kidney. The prominent staining of SAH hydrolase in the podocytes may reflect high rates of transmethylation. (J Histochem Cytochem 48:211–218, 2000)

Key Words: S-adenosylhomocysteine hydrolase, enzyme activity, immunohistochemistry, glomerulus, podocyte, confocal LSM, fluorescence


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