Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Niemann, A.
Right arrow Articles by Elsässer, H.-P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Niemann, A.
Right arrow Articles by Elsässer, H.-P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 48, 251-258, February 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

The Lysosomotropic Agent Monodansylcadaverine Also Acts as a Solvent Polarity Probe

Axel Niemanna, Akira Takatsukib, and Hans-Peter Elsässera
a Department of Cell Biology, University of Marburg, Marburg, Germany
b Saitama, Japan

Correspondence to: Hans-Peter Elsässer, Robert-Koch-Str. 5, Marburg, Germany. E-mail: elsaesse@mailer.uni-marburg.de

The autofluorescent substance monodansylcadaverine has recently been reported as a specific in vivo marker for autophagic vacuoles. However, the mechanism for this specific labeling remained unclear. Our results reveal that the common model of ion trapping in acidic compartments cannot completely account for the observed autophagic vacuole staining. Because autophagic vacuoles are characterized by myelin-like membrane inclusions, we tested whether this lipid-rich environment is responsible for the staining properties of monodansylcadaverine. In in vitro experiments using either liposomes or solvents of different polarity, monodansylcadaverine showed an increased relative fluorescence intensity in a hydrophobic environment as well as a Stokes shift dependent on the solvent polarity. To test the effect of autophagic vacuoles or autophagic vacuole lipids on monodansylcadaverine fluorescence, we isolated autophagic vacuoles and purified autophagic vacuole lipids depleted of proteins. Entire autophagic vacuoles and autophagic vacuole lipids had the same effect on monodansylcadaverine fluorescence properties, suggesting lipids as the responsible component. Our results suggest that the in vivo fluorescence properties of monodansylcadaverine do not depend exclusively on accumulation in acidic compartments by ion trapping but also on an effective interaction of this molecule with autophagic vacuole membrane lipids. (J Histochem Cytochem 48:251–258, 2000)

Key Words: monodansylcadaverine, lysosomotropic agent, autophagic vacuole, solvent polarity probe, Stokes shift


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Cancer Res.Home page
A. Beckers, S. Organe, L. Timmermans, K. Scheys, A. Peeters, K. Brusselmans, G. Verhoeven, and J. V. Swinnen
Chemical Inhibition of Acetyl-CoA Carboxylase Induces Growth Arrest and Cytotoxicity Selectively in Cancer Cells
Cancer Res., September 1, 2007; 67(17): 8180 - 8187.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
J.-h. Zhu, C. Horbinski, F. Guo, S. Watkins, Y. Uchiyama, and C. T. Chu
Regulation of Autophagy by Extracellular Signal-Regulated Protein Kinases During 1-Methyl-4-Phenylpyridinium-Induced Cell Death
Am. J. Pathol., January 1, 2007; 170(1): 75 - 86.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
W. Martinet, G. R.Y. De Meyer, L. Andries, A. G. Herman, and M. M. Kockx
In Situ Detection of Starvation-induced Autophagy
J. Histochem. Cytochem., January 1, 2006; 54(1): 85 - 96.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
P. Lajoie, G. Guay, J. W. Dennis, and I. R. Nabi
The lipid composition of autophagic vacuoles regulates expression of multilamellar bodies
J. Cell Sci., May 1, 2005; 118(9): 1991 - 2003.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. L. Styers, G. Salazar, R. Love, A. A. Peden, A. P. Kowalczyk, and V. Faundez
The Endo-Lysosomal Sorting Machinery Interacts with the Intermediate Filament Cytoskeleton
Mol. Biol. Cell, December 1, 2004; 15(12): 5369 - 5382.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. P. Otto, M. Y. Wu, N. Kazgan, O. R. Anderson, and R. H. Kessin
Macroautophagy Is Required for Multicellular Development of the Social Amoeba Dictyostelium discoideum
J. Biol. Chem., May 9, 2003; 278(20): 17636 - 17645.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
B. Ravikumar, A. Stewart, H. Kita, K. Kato, R. Duden, and D. C. Rubinsztein
Raised intracellular glucose concentrations reduce aggregation and cell death caused by mutant huntingtin exon 1 by decreasing mTOR phosphorylation and inducing autophagy
Hum. Mol. Genet., May 1, 2003; 12(9): 985 - 994.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
B. Inbal, S. Bialik, I. Sabanay, G. Shani, and A. Kimchi
DAP kinase and DRP-1 mediate membrane blebbing and the formation of autophagic vesicles during programmed cell death
J. Cell Biol., April 29, 2002; 157(3): 455 - 468.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
A. Petersen, K. E. Larsen, G. G. Behr, N. Romero, S. Przedborski, P. Brundin, and D. Sulzer
Expanded CAG repeats in exon 1 of the Huntington's disease gene stimulate dopamine-mediated striatal neuron autophagy and degeneration
Hum. Mol. Genet., June 1, 2001; 10(12): 1243 - 1254.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
A. Niemann, J. Baltes, and H.-P. Elsässer
Fluorescence Properties and Staining Behavior of Monodansylpentane, a Structural Homologue of the Lysosomotropic Agent Monodansylcadaverine
J. Histochem. Cytochem., February 1, 2001; 49(2): 177 - 186.
[Abstract] [Full Text]

Home page
 JCBHome page
B. Inbal, S. Bialik, I. Sabanay, G. Shani, and A. Kimchi
DAP kinase and DRP-1 mediate membrane blebbing and the formation of autophagic vesicles during programmed cell death
J. Cell Biol., April 29, 2002; 157(3): 455 - 468.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2000

 
Purchase HCS Short Course Manual on HCS site