Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McGinley, J. N.
Right arrow Articles by Thompson, H. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McGinley, J. N.
Right arrow Articles by Thompson, H. J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 48, 355-362, March 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

Effect of Fixation and Epitope Retrieval on BrdU Indices in Mammary Carcinomas

John N. McGinleya, Katrina K. Knotta, and Henry J. Thompsona
a AMC Cancer Research Center, Denver, Colorado

Correspondence to: John N. McGinley, AMC Cancer Research Center, 1600 Pierce St., Denver, CO 80214. E-mail: mcginleyj@amc.org

Studies in which 5-bromo-2'-deoxyuridine (BrdU) is used to quantify rates of cell proliferation are conducted prospectively. Therefore, the opportunity exists to select conditions that optimize detection of the BrdU epitope. The objective of this study was to quantify the extent to which the BrdU epitope was masked by formalin vs methacarn fixation in the assessment of cell proliferation. Mammary carcinomas from animals pulse-labeled with BrdU were trisected. A portion was frozen and the remaining two portions were fixed in 10% neutral buffered formalin or methacarn for 24 hr, processed, embedded in paraffin, and sections stained for incorporated BrdU using a peroxidase immunohistochemical staining technique. Antigen retrieval techniques also were applied to formalin-fixed sections. Fixation in methacarn gave the highest labeling index (16.4%), which was comparable to that observed in unfixed frozen sections (17.5%). Formalin fixation alone dramatically suppressed the labeling index (0.3%), which was only partially recovered using various antigen retrieval techniques (2.1–8.1%). Methacarn fixation is recommended for prospective studies in which BrdU detection is planned because of the quantitative recovery of epitope and the simplicity of the approach. (J Histochem Cytochem 48:355–362, 2000)

Key Words: immunohistochemistry, bromodeoxyuridine, antigen retrieval, enzyme digestion, formalin, methacarn, fixation, image analysis, microwave


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. T. Foster and T. J. Bartness
Sympathetic but not sensory denervation stimulates white adipocyte proliferation
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2006; 291(6): R1630 - R1637.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
L. Wu and I. F Tannock
Repopulation in Murine Breast Tumors during and after Sequential Treatments with Cyclophosphamide and 5-Fluorouracil
Cancer Res., May 1, 2003; 63(9): 2134 - 2138.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
A. Pernthaler, J. Pernthaler, M. Schattenhofer, and R. Amann
Identification of DNA-Synthesizing Bacterial Cells in Coastal North Sea Plankton
Appl. Envir. Microbiol., November 1, 2002; 68(11): 5728 - 5736.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
S. M. Swanson and T. G. Unterman
The growth hormone-deficient Spontaneous Dwarf rat is resistant to chemically induced mammary carcinogenesis
Carcinogenesis, June 1, 2002; 23(6): 977 - 982.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
J. N. McGinley, K. K. Knott, and H. J. Thompson
Semi-automated Method of Quantifying Vasculature of 1-Methyl-1-nitrosourea-induced Rat Mammary Carcinomas Using Immunohistochemical Detection
J. Histochem. Cytochem., February 1, 2002; 50(2): 213 - 222.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
C. R. Madden, M. J. Finegold, and B. L. Slagle
Hepatitis B Virus X Protein Acts as a Tumor Promoter in Development of Diethylnitrosamine-Induced Preneoplastic Lesions
J. Virol., April 15, 2001; 75(8): 3851 - 3858.
[Abstract] [Full Text]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2000