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Journal of Histochemistry and Cytochemistry, Vol. 48, 415-422, March 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

Expression of Glutamine Synthetase in Macrophages

Johannes Georg Bodea, Thorsten Peters–Regehra, Ralf Kubitza, and Dieter Häussingera
a Medizinische Universitätsklinik, Klinik für Gastroenterologie, Hepatologie und Infektiologie, Heinrich-Heine Universität, Düsseldorf, Germany

Correspondence to: Dieter Häussinger, Medizinische Universitätsklinik, Klinik für Gastroenterologie, Hepatologie und Infektiologie, Heinrich-Heine Universität, Moorenstr. 5, D-40225 Düsseldorf, Germany.

We studied the expression of glutamine synthetase in liver macrophages (Kupffer cells, KCs) in situ and in culture. Glutamine synthetase was detectable at the mRNA and protein level in freshly isolated and short-term-cultured rat liver macrophages. Enzyme activity and protein content were about 9% of that in liver parenchymal cells. In contrast, glutamine synthetase mRNA levels in liver macrophages apparently exceeded those in parenchymal liver cells (PCs). By use of confocal laser scanning microscopy and specific macrophage markers, immunoreactive glutamine synthetase was localized to macrophages in normal rat liver and normal human liver in situ. All liver macrophages stained positive for glutamine synthetase. In addition, macrophages in rat pancreas contained immunoreactive glutamine synthetase, whereas glutamine synthetase was not detectable at the mRNA and protein level in blood monocytes and RAW 264.7 mouse macrophages. No significant amounts of glutamine synthetase were found in isolated rat liver sinusoidal endothelial cells (SECs). The data suggest a constitutive expression of glutamine synthetase not only, as previously believed, in perivenous liver parenchymal cells but also in resident liver macrophages. (J Histochem Cytochem 48:415–421, 2000)

Key Words: glutamine metabolism, sinusoidal endothelial cells, monocytes


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