Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hainfeld, J. F.
Right arrow Articles by Powell, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hainfeld, J. F.
Right arrow Articles by Powell, R. D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 48, 471-480, April 2000, Copyright © 2000, The Histochemical Society, Inc.

SYMPOSIUM

New Frontiers in Gold Labeling

James F. Hainfelda and Richard D. Powellb
a Biology Department, Brookhaven National Laboratory, Upton, New York
b Nanoprobes, Inc., Stony Brook, New York

Correspondence to: James F. Hainfeld, Biology Department, Brookhaven National Laboratory, Upton, NY 11973. E-mail: hainfeld@bnl.gov

Recent advances in gold technology have led to probes with improved properties and performance for cell biologists: higher labeling density, better sensitivity, and greater penetration into tissues. Gold clusters, such as the 1.4-nm Nanogold, are gold compounds that can be covalently linked to Fab' antibody fragments, making small and stable probes. Silver enhancement then makes these small gold particles easily visible by EM, LM, and directly by eye. Another advance is the combination of fluorescent and gold probes for correlative microscopy. Chemical crosslinking of gold particles to many biologically active molecules has made possible many novel probes, such as gold–lipids, gold–Ni–NTA, and gold–ATP. (J Histochem Cytochem 48: 471–480, 2000)

Key Words: gold, immunogold, electron microscopy, gold labeling, gold clusters, immunocytochemistry, colloidal gold, Nanogold


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
R. A. Coleman, J. Liu, and J. B. Wade
Use of Anti-fluorophore Antibody to Achieve High-sensitivity Immunolocalizations of Transporters and Ion Channels
J. Histochem. Cytochem., July 1, 2006; 54(7): 817 - 827.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. L. Wolfe, J. A. Warrington, L. Treadwell, and M. T. Norcum
A Three-dimensional Working Model of the Multienzyme Complex of Aminoacyl-tRNA Synthetases Based on Electron Microscopic Placements of tRNA and Proteins
J. Biol. Chem., November 18, 2005; 280(46): 38870 - 38878.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
T. Cheutin, M.-F. O'Donohue, A. Beorchia, C. Klein, H. Kaplan, and D. Ploton
Three-dimensional Organization of pKi-67: A Comparative Fluorescence and Electron Tomography Study Using Fluoronanogold
J. Histochem. Cytochem., November 1, 2003; 51(11): 1411 - 1423.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Cognet, C. Tardin, D. Boyer, D. Choquet, P. Tamarat, and B. Lounis
Single metallic nanoparticle imaging for protein detection in cells
PNAS, September 30, 2003; 100(20): 11350 - 11355.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
N.-J. Xu, L. Bao, H.-P. Fan, G.-B. Bao, L. Pu, Y.-J. Lu, C.-F. Wu, X. Zhang, and G. Pei
Morphine Withdrawal Increases Glutamate Uptake and Surface Expression of Glutamate Transporter GLT1 at Hippocampal Synapses
J. Neurosci., June 1, 2003; 23(11): 4775 - 4784.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
Y. Xiao, F. Patolsky, E. Katz, J. F. Hainfeld, and I. Willner
"Plugging into Enzymes": Nanowiring of Redox Enzymes by a Gold Nanoparticle
Science, March 21, 2003; 299(5614): 1877 - 1881.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
D. Boyer, P. Tamarat, A. Maali, B. Lounis, and M. Orrit
Photothermal Imaging of Nanometer-Sized Metal Particles Among Scatterers
Science, August 16, 2002; 297(5584): 1160 - 1163.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. Cheutin, M.-F. O'Donohue, A. Beorchia, M. Vandelaer, H. Kaplan, B. Defever, D. Ploton, and M. Thiry
Three-dimensional organization of active rRNA genes within the nucleolus
J. Cell Sci., August 15, 2002; 115(16): 3297 - 3307.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2000

 
Purchase HCS Short Course Manual on HCS site