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Journal of Histochemistry and Cytochemistry, Vol. 48, 487-492, April 2000, Copyright © 2000, The Histochemical Society, Inc.

SYMPOSIUM

Enhanced Labeling Efficiency Using Ultrasmall Immunogold Probes: Immunocytochemistry

John M. Robinsona, Toshihiro Takizawab, and Dale D. Vandréa
a Department of Physiology and Cell Biology, Ohio State University, Columbus, Ohio
b Department of Anatomy, Jichi Medical School, Tochigi, Japan

Correspondence to: John M. Robinson, Dept. of Physiology and Cell Biology, Ohio State U., 302 Hamilton Hall, 1645 Neil Ave., Columbus, OH 43210. E-mail: robinson.21@osu.edu

Detection of antigen–antibody interactions in immunocytochemistry relies on a reporter system. The most commonly employed reporter systems used are fluorochromes, enzymes, and particulate probes. This article considers the advantages and disadvantages associated with ultrasmall immunogold particles as the reporter system in immunocytochemical applications. (J Histochem Cytochem 48:487–492, 2000)

Key Words: ultrasmall immunogold, Nanogold, FluoroNanogold, microtubules, centrosomes


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