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Journal of Histochemistry and Cytochemistry, Vol. 48, 535-544, April 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

Human Bronchial Epithelial Cells Secrete Laminin 5, Express Hemidesmosomal Proteins, and Assemble Hemidesmosomes

Peter H. Michelsona,b, Margaret Tiguea, and Jonathan C.R. Jonesc
a Departments of Pediatrics, Northwestern University Medical School, Chicago, Iillinois
b Medicine, Northwestern University Medical School, Chicago, Iillinois
c Cell and Molecular Biology, Northwestern University Medical School, Chicago, Iillinois

Correspondence to: Peter H. Michelson, Div. of Pulmonary and Critical Care Medicine, Northwestern U. Medical School, 300 E. Superior, Tarry 14 - # 707, Chicago, IL 60611. E-mail: pmichelson@nwu.edu

Epithelial cells attach to the basement membrane through adhesive contacts between the basal cells of the epithelium and the proteins of the extracellular matrix (ECM). The hemidesmosome (HD) is a specialized cell–ECM contact, that mediates the attachment of the epithelial cell basal surface to the ECM. In bronchial epithelial cells, the protein components that constitute the HD have not been demonstrated. Using immunohistochemical techniques, we determined that normal human bronchial epithelial (NHBE) cells express the HD cell surface integrin {alpha}6ß4 and produce laminin 5, the ECM protein associated with HDs. Furthermore, expression of the HD-associated structural proteins, bullous pemphigoid antigens 1 (BPAG 1) and 2 (BPAG 2), was demonstrated in NHBE cells by immunofluorescence microscopy and immunoblot analyses. In addition, we confirmed the presence of laminin 5 in the basement membrane (BM) of bronchial epithelial biopsy specimens and of BP230, BP180, and the {alpha}6ß4 integrin heterodimer at the site of bronchial epithelial cell–ECM interaction in vivo. Finally, using electron microscopy, we were able to demonstrate intact HDs in a glutaraldehyde-fixed NHBE cell monolayer. These findings suggest that bronchial epithelium forms HDs and that the laminin 5–{alpha}6ß4 integrin interaction may be important in stabilizing epithelial cell adhesion to the BM in the lung. (J Histochem Cytochem 48:535–544, 2000)

Key Words: hemidesmosome (HD), integrin, extracellular matrix (ECM)


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