Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sheinin, Y.
Right arrow Articles by Cross, H. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sheinin, Y.
Right arrow Articles by Cross, H. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 48, 595-602, May 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

Immunocytochemical Localization of the Extracellular Calcium-Sensing Receptor in Normal and Malignant Human Large Intestinal Mucosa

Yuri Sheinina, Enikö Kállaya, Friedrich Wrbab, Stefan Kriwanekc, Meinrad Peterlika, and Heide S. Crossa
a Departments of General and Experimental Pathology, Vienna, Austria
b Clinical Pathology, Vienna, Austria
c University of Vienna Medical School, General Hospital, and Krankenhaus Rudolfsstiftung, Department of Surgery I, Vienna, Austria

Correspondence to: Heide S. Cross, Dept. of General and Experimental Pathology, Waehringer Guertel 18–20, A-1090 Vienna, Austria. E-mail: heide.cross@akh-wien.ac.at

We identified the parathyroid type Ca2+-sensing receptor (CaR) in normal human colon mucosa and in cancerous lesions at the mRNA and protein level. Polymerase chain reaction produced an amplification product from reverse-transcribed large intestinal RNA which corresponded in size and length to a 537-bp sequence from exon 7 of the CaR gene. With a specific antiserum against its extracellular domain, the CaR could be detected by immunostaining in normal human colon mucosa in cells preferentially located at the crypt base. The CaR protein was also expressed in tumors of the large bowel in all 20 patients examined. However, the great majority of CaR-positive cells in the adenocarcinomas inspected were confined to more differentiated areas exhibiting glandular–tubular structures. Poorly or undifferentiated regions were either devoid of specific immunoreactivity or contained only isolated CaR-positive cells. In the normal mucosa and in glandular–tubular structures of cancerous lesions, the CaR was exclusively expressed in chromogranin A-positive enteroendocrine cells and in only a small fraction of PCNA-positive cells. (J Histochem Cytochem 48:595–601, 2000)

Key Words: calcium-sensing receptor, growth control, colorectal cancer, chromogranin A, endocrine cells, PCNA, differentiation, immunohistochemistry, reverse transcription, PCR


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
D. Peiris, I. Pacheco, C. Spencer, and R. J. MacLeod
The extracellular calcium-sensing receptor reciprocally regulates the secretion of BMP-2 and the BMP antagonist Noggin in colonic myofibroblasts
Am J Physiol Gastrointest Liver Physiol, March 1, 2007; 292(3): G753 - G766.
[Abstract] [Full Text] [PDF]

Home page
 JNCI J Natl Cancer InstHome page
M. V. Grau, J. A. Baron, R. S. Sandler, K. Wallace, R. W. Haile, T. R. Church, G. J. Beck, R. W. Summers, E. L. Barry, B. F. Cole, et al.
Prolonged Effect of Calcium Supplementation on Risk of Colorectal Adenomas in a Randomized Trial
J Natl Cancer Inst, January 17, 2007; 99(2): 129 - 136.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. Liao, A. Schneider, N. S. Datta, and L. K. McCauley
Extracellular calcium as a candidate mediator of prostate cancer skeletal metastasis.
Cancer Res., September 15, 2006; 66(18): 9065 - 9073.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Geibel, K. Sritharan, R. Geibel, P. Geibel, J. S. Persing, A. Seeger, T. K. Roepke, M. Deichstetter, C. Prinz, S. X. Cheng, et al.
Inaugural Article: Calcium-sensing receptor abrogates secretagogue- induced increases in intestinal net fluid secretion by enhancing cyclic nucleotide destruction
PNAS, June 20, 2006; 103(25): 9390 - 9397.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. Chakrabarty, H. Wang, L. Canaff, G. N. Hendy, H. Appelman, and J. Varani
Calcium Sensing Receptor in Human Colon Carcinoma: Interaction with Ca2+ and 1,25-Dihydroxyvitamin D3
Cancer Res., January 15, 2005; 65(2): 493 - 498.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
U. Peters, N. Chatterjee, M. Yeager, S. J. Chanock, R. E. Schoen, K. A. McGlynn, T. R. Church, J. L. Weissfeld, A. Schatzkin, and R. B. Hayes
Association of Genetic Variants in the Calcium-Sensing Receptor with Risk of Colorectal Adenoma
Cancer Epidemiol. Biomarkers Prev., December 1, 2004; 13(12): 2181 - 2186.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. Chakrabarty, V. Radjendirane, H. Appelman, and J. Varani
Extracellular Calcium and Calcium Sensing Receptor Function in Human Colon Carcinomas: Promotion of E-Cadherin Expression and Suppression of {beta}-Catenin/TCF Activation
Cancer Res., January 1, 2003; 63(1): 67 - 71.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. X. Cheng, M. Okuda, A. E. Hall, J. P. Geibel, and S. C. Hebert
Expression of calcium-sensing receptor in rat colonic epithelium: evidence for modulation of fluid secretion
Am J Physiol Gastrointest Liver Physiol, July 1, 2002; 283(1): G240 - G250.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2000