Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Van der Gucht, E.
Right arrow Articles by Arckens, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Van der Gucht, E.
Right arrow Articles by Arckens, L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 48, 671-684, May 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

A New Cat Fos Antibody to Localize the Immediate Early Gene c-fos in Mammalian Visual Cortex after Sensory Stimulation

Estel Van der Guchta,c, Erik Vandenbusscheb, Guy A. Orbanc, Frans Vandesandea, and Lutgarde Arckensa,c
a Laboratory of Neuroendocrinology and Immunological Biotechnology, Katholieke Universiteit Leuven
b Laboratory of Neuropsychology, Medical School, Katholieke Universiteit Leuven, Leuven, Belgium
c Laboratorium voor Neuro- en Psychofysiologie, Medical School, Katholieke Universiteit Leuven, Leuven, Belgium

Correspondence to: Lutgarde Arckens, Laboratory of Neuroendocrinology and Immunological Biotechnology, Katholieke Uni-versiteit Leuven, Naamsestr. 59, B-3000 Leuven, Belgium. E-mail: Lut.Arckens@bio.kuleuven.ac.be

We developed a novel antibody against cat Fos by immunizing rabbits with a 26-amino-acid peptide. Immunocytochemistry on visual cortex of cats undergoing different visual manipulations was applied to test the reliability and the efficacy of this antiserum. One hour of light stimulation after an overnight dark adaptation resulted in strongly induced Fos expression in supra- and infragranular layers of cat primary visual cortex. Short-term monocular deprivation changed the Fos expression profile into a columnar immu-nostaining related to ocular dominance columns. Fos expression has also been analyzed in cats in which visual input was confined to the right hemisphere by sectioning the left optic tract and the corpus callosum. In the right hemisphere, visual stimulation elicited Fos induction, whereas in the contralateral hemisphere a very low Fos signal was observed. The specificity of this newly synthesized antibody was confirmed by Western blotting. To further establish the applicability of this Fos antiserum, we performed immunostaining on monkey and rat visual cortex. This new cat Fos antibody appears to be excellent for study of Fos expression as a marker for mapping neuronal activity in mammalian brain. (J Histochem Cytochem 48:671–684, 2000)

Key Words: immediate early gene (IEG), antibody production, Western blotting, immunocytochemistry, Fos expression, visual cortex, cat, monkey


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Cereb CortexHome page
L. Cnops, T.-T. Hu, K. Burnat, and L. Arckens
Influence of Binocular Competition on the Expression Profiles of CRMP2, CRMP4, Dyn I, and Syt I in Developing Cat Visual Cortex
Cereb Cortex, May 1, 2008; 18(5): 1221 - 1231.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
E. Van der Gucht, P. R. Hof, L. Van Brussel, K. Burnat, and L. Arckens
Neurofilament Protein and Neuronal Activity Markers Define Regional Architectonic Parcellation in the Mouse Visual Cortex
Cereb Cortex, December 1, 2007; 17(12): 2805 - 2819.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
D. Granados-Fuentes, A. Tseng, and E. D. Herzog
A Circadian Clock in the Olfactory Bulb Controls Olfactory Responsivity.
J. Neurosci., November 22, 2006; 26(47): 12219 - 12225.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2000