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Journal of Histochemistry and Cytochemistry, Vol. 48, 685-694, May 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

Immunohistochemical Localization of cGMP-binding cGMP-specific Phosphodiesterase (PDE5) in Rat Tissues

Jun Koteraa, Kotomi Fujishigea, and Kenji Omoria
a Discovery Research Laboratory, Tanabe Seiyaku Co., Ltd., Saitama, Japan

Correspondence to: Kenji Omori, Discovery Research Laboratory, Tanabe Seiyaku Co., Ltd., 2-50, Kawagishi-2-chome, Toda, Saitama 335-8505, Japan.

We raised a polyclonal antibody against maltose binding protein fusion human cGMP-binding, cGMP-specific phosphodiesterase (PDE5) produced in E. coli. This antibody immunoreacted specifically with recombinant human and rat PDE5 proteins expressed in transfected COS-7 cells and with a native form of PDE5 in extracts of rat platelets, lung, and cerebellum. Immunohistochemical analysis showed that the anti-PDE5 antibody detected immunoactive materials in Purkinje cell layers of the cerebellum, proximal renal tubules, collecting renal ducts, and epithelial cells of pancreatic ducts in rats. Reverse transcriptase-polymerase chain reaction analysis demonstrated that PDE5 transcripts are also present in rat cerebellum, kidney, and pancreas. Here we described a cell-specific localization of PDE5 in various rat tissues, suggesting the possibility of the presence of a cGMP/PDE5 pathway in these tissues. (J Histochem Cytochem 48:685–693, 2000)

Key Words: cGMP, phosphodiesterase, cerebellum, kidney, pancreas


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