Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hayashi, H.
Right arrow Articles by Onozuka, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hayashi, H.
Right arrow Articles by Onozuka, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 48, 695-698, May 2000, Copyright © 2000, The Histochemical Society, Inc.

ARTICLE

Morphological Aspects of the Postnatal Development of Submandibular Glands in Male Rats: Involvement of Apoptosis

Hiroyuki Hayashia, Satoru Ozonob, Kazuko Watanabec, Ikuko Nagatsue, and Minoru Onozukad
a Departments of Oral Histology, Kanagawa Dental College, Yokosuka, Japan
b Pathology, Kanagawa Dental College, Yokosuka, Japan
c Departments of Physiology, Gifu University School of Medicine, Gifu, Japan
d Anatomy, Gifu University School of Medicine, Gifu , Japan
e Department of Anatomy, Fujita Health University School of Medicine, Toyoake, Japan

Correspondence to: Minoru Onozuka, Dept. of Anatomy (2nd Division), Gifu University School of Medicine, 40 Tsukasa-machi, Gifu 500-8705, Japan. E-mail: onozuka@cc.gifu-u.ac.jp

We studied the involvement of the apoptotic mechanism(s) in cell differentiation in the developing male rat submandibular gland using the TUNEL (terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate–biotin nick-labeling) assay in combination with light and electron microscopy. Whereas the proacinar cells were completely transformed into acinar cells within 2 weeks after birth, starting on postnatal Day 21, the terminal tubule cells formed vacuoles that disappeared by postnatal Day 35. During this period, positive TUNEL reactivity was seen in the terminal tubule cells, and electron microscopic analysis showed that certain morphological features of apoptosis, including fragmentation of nuclei and the presence of apoptotic bodies in the cytoplasm, were present in and restricted to the terminal tubule cells. These results indicate that, in addition to an autophagocytosis-mediated mechanism, apoptosis may also be involved in reducing the number of terminal tubule cells during postnatal development in the submandibular gland. (J Histochem Cytochem 48:695–698, 2000)

Key Words: apoptosis, development, cell differentiation, terminal tubule cells, submandibular gland, TUNEL staining


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2000